Salidroside impedes Ang II-infused myocardial fibrosis by activating the SIRT1-Nrf2 pathway.

IF 2.1 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Iranian Journal of Basic Medical Sciences Pub Date : 2025-01-01 DOI:10.22038/ijbms.2025.83659.18105

Xi Zhu, Zhen Hai, Zhongping Ning

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Abstract

Objectives: This research examined the protective function of salidroside (SAL) against angiotensin II (Ang II)-infused myocardial fibrosis and its associated mechanism.

Materials and methods: The C57BL/6 male murine models (n=24) received either saline solution or Ang II (1500 ng/kg/day) subcutaneously and an oral dosage of SAL (50 mg/kg/day) once daily for 28 days. Newborn Sprague-Dawley (SD) rats were used to isolate atrial fibroblasts.

Results: The fibrotic region was raised by Ang II infusion, while SAL treatment inhibited it. Collagen I and III expression was raised by Ang II induction, but SAL therapy reduced their expression. SAL therapy also decreased the expression of other fibroblast differentiation-related markers induced by Ang II infusion. It elevated SIRT1, Nrf2, and HO-1 levels in atrial fibroblasts. Additionally, SAL significantly inhibited atrial fibroblasts, whereas EX527, an inhibitor of SIRT1, noticeably increased the migration ability. Furthermore, SAL suppressed intracellular ROS production and oxidative stress in Ang II-infused atrial fibroblasts.

Conclusion: SAL protects against myocardial fibrosis infused by Ang II by activating the SIRT1-Nrf2 pathway.

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红景天苷通过激活SIRT1-Nrf2通路阻碍angii灌注心肌纤维化。

目的：探讨红景天苷（SAL）对血管紧张素II （Ang II）灌注心肌纤维化的保护作用及其机制。材料与方法：C57BL/6雄性小鼠模型（n=24）分别皮下注射生理盐水或angii (1500 ng/kg/d)，同时口服SAL (50 mg/kg/d)，每日1次，连用28 d。采用新生SD大鼠分离心房成纤维细胞。结果：Angⅱ输注使纤维化区升高，SAL对其有抑制作用。angii诱导可提高I和III型胶原的表达，但SAL治疗可降低其表达。SAL治疗还降低了Ang II输注诱导的其他成纤维细胞分化相关标志物的表达。升高心房成纤维细胞中SIRT1、Nrf2和HO-1水平。此外，SAL显著抑制心房成纤维细胞，而SIRT1抑制剂EX527显著增加迁移能力。此外，SAL抑制了angii灌注心房成纤维细胞内ROS的产生和氧化应激。结论：SAL通过激活SIRT1-Nrf2通路，对angii输注的心肌纤维化具有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY

CiteScore

4.00

自引率

4.50%

发文量

142

审稿时长

6-12 weeks

期刊介绍： The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.