Group-specific Quantification of mcrA genes of Methanogenic Archaea and "Candidatus Methanoperedens" by Digital PCR.

IF 2 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbes and Environments Pub Date : 2025-01-01 DOI:10.1264/jsme2.ME24097

Takeshi Watanabe, Atsuya Endo, Rio Hamada, Rina Shinjo, Susumu Asakawa

引用次数: 0

Abstract

Digital PCR is a technique that quantifies target genes based on the absence or presence of the targets in PCR amplicons. The present study exami-ned group-specific probes for the quantification of mcrA genes in six methanogenic archaeal groups and "Candidatus Methanoperedens" by digital PCR with the universal primers ML-f and ML-r. A digital PCR ana-lysis of paddy field soil detected all the targets, with the dominant and minor groups being Methanomicrobiales and Methanobrevibacter spp., respectively (10⁷ and 10⁴ copies [g dry soil]^-1). This method has the potential to reveal the dynamics of specific methanogenic archaeal groups in the environment.

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产甲烷古菌和“Methanoperedens Candidatus Methanoperedens”mcrA基因的群体特异性定量分析。

数字PCR是一种基于PCR扩增子中目标基因的缺失或存在来量化目标基因的技术。本研究以通用引物ML-f和ML-r为引物，对6个产甲烷古菌群和“Candidatus Methanoperedens”的mcrA基因进行了群体特异性PCR检测。对水田土壤进行数字PCR分析，检测到所有目标菌，优势群和次要群分别为Methanomicrobiales和Methanobrevibacter spp.（107和104拷贝[g干土]-1）。这种方法有可能揭示环境中特定产甲烷古菌群的动态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.