H. pylori infection downregulates the expression and release of miR- 223 in neutrophils.

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Microbiology Pub Date : 2025-10-01 Epub Date: 2025-04-11 DOI:10.1007/s10123-025-00660-9

Yu Zhang, Chang Xu, Hao-Ming Zhong, Yu Song, Hong Luo, Peng Liu

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引用次数: 0

Abstract

Objective: This study aimed to investigate the association between serum miR- 223 concentration and Helicobacter pylori (H. pylori) infection.

Methods: H. pylori status was assessed using the Urea ¹³C Breath Test Kit and Typing Detection Kit for antibodies against H. pylori. Patients were considered positive for H. pylori infection when both tests yielded positive results. Serum miRNAs were extracted using the miRNeasy Mini Kit, and quantitative real-time PCR was performed to analyze the relative expression level of miR- 223.

Results: We found that the relative expression level of miR- 223 in neutrophils from H. pylori-positive patients (20.35 ± 5.85) was significantly lower than that from healthy individuals (45.92 ± 10.59) (p < 0.05). Moreover, the expression level of miR- 7, which we selected as a control molecule, was not significantly lower in neutrophils from H. pylori-positive patients (3.07 ± 0.78) than healthy controls (4.43 ± 1.57) (p > 0.05), and the expression of miR- 7 was lower than miR- 223.

Conclusion: These results indicated that circulating miR- 223 down-expression was of neutrophil origin in vitro and inversely associated with H. pylori infection.

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幽门螺杆菌感染可下调中性粒细胞miR- 223的表达和释放。

目的：探讨血清miR- 223浓度与幽门螺杆菌感染的关系。方法：采用尿素13C呼气测试试剂盒和幽门螺杆菌抗体分型检测试剂盒评估幽门螺杆菌状态。当两项检测结果均为阳性时，则认为患者幽门螺杆菌感染呈阳性。使用miRNeasy Mini Kit提取血清mirna，采用实时荧光定量PCR分析miR- 223的相对表达水平。结果：miR- 223在幽门螺杆菌阳性患者中性粒细胞中的相对表达量（20.35±5.85）明显低于正常人群（45.92±10.59）（p < 0.05）。此外，我们选择作为对照分子的miR- 7在幽门螺杆菌阳性患者中性粒细胞中的表达水平（3.07±0.78）并不显著低于健康对照组（4.43±1.57）（p > 0.05）， miR- 7的表达低于miR- 223。结论：体外循环miR- 223表达下调与幽门螺杆菌感染呈负相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Microbiology 生物-生物工程与应用微生物

CiteScore

5.50

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： International Microbiology publishes information on basic and applied microbiology for a worldwide readership. The journal publishes articles and short reviews based on original research, articles about microbiologists and their work and questions related to the history and sociology of this science. Also offered are perspectives, opinion, book reviews and editorials. A distinguishing feature of International Microbiology is its broadening of the term microbiology to include eukaryotic microorganisms.