Lactiplantibacillus plantarum as a sustainable solution for monocrotophos degradation and plant growth enhancement.

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Microbiology Pub Date : 2025-05-07 DOI:10.1007/s10123-025-00671-6

Archana Kumari, Chiranjit Ghosh, N Kannan, S Balaji

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Abstract

In the present study, characterization of bacterial isolate from fermented foods based on the morphology, biochemical, and molecular analysis identified Lactiplantibacillus plantarum. The bacterium was tested for its ability to degrade monocrotophos (MCP) at a concentration of 1000 ppm. The dephosphorylation potential of this bacterial enzyme against organophosphate substrates including MCP was analyzed. The Lactiplantibacillus plantarum phosphatase showed optimal activity at 40 °C and 6 pH. The Michaelis-Menten constant was found to be 238.14 µM while Vmax is 357.14 µmol/min.ml for MCP as a substrate. The enzyme activity was enhanced by metal ions such as Mn²⁺, Mg²⁺, and Ca²⁺ and inhibited by K⁺, Zn²⁺, and Fe²⁺ ions. Plant growth promotion assays of Lp. plantarum exhibited characteristic traits including phosphate solubilization, cellulase, catalase, and xylanase activities. The degradation of MCP was confirmed based on the presence of acetamide and trimethyl phosphate as intermediate metabolites, confirmed by FTIR and GC-MS analysis. Thus, L. plantarum is a suitable candidate for phosphate solubilization, plant growth promotion, and bio-fertilizer application.

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植物乳酸菌作为一种可持续的解决方案，降解和促进植物生长。

本研究从发酵食品中分离出一株植物乳杆菌，通过形态学、生化和分子分析对其进行鉴定。测试了该细菌在1000ppm浓度下降解单效磷（MCP）的能力。分析了该细菌酶对包括MCP在内的有机磷底物的去磷酸化电位。植物乳杆菌磷酸酶在40℃、6 ph条件下活性最佳，Michaelis-Menten常数为238.14µmol/min， Vmax为357.14µmol/min。MCP作为底物。金属离子Mn2+、Mg2+和Ca2+增强了酶活性，K+、Zn2+和Fe2+抑制了酶活性。植物生长促进试验。植物表现出磷酸盐增溶、纤维素酶、过氧化氢酶和木聚糖酶活性等特征。通过FTIR和GC-MS分析，证实了MCP的降解过程中存在乙酰胺和磷酸三甲酯作为中间代谢产物。因此，植物乳杆菌是磷肥增溶剂、植物生长促进剂和生物肥料的适宜候选者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Microbiology 生物-生物工程与应用微生物

CiteScore

5.50

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： International Microbiology publishes information on basic and applied microbiology for a worldwide readership. The journal publishes articles and short reviews based on original research, articles about microbiologists and their work and questions related to the history and sociology of this science. Also offered are perspectives, opinion, book reviews and editorials. A distinguishing feature of International Microbiology is its broadening of the term microbiology to include eukaryotic microorganisms.