circ_0006168 expression serves as a potential biomarker of acquired middle ear cholesteatoma.

IF 2.2 3区 医学 Q2 OTORHINOLARYNGOLOGY
Chen Liu, Bo Ning, Bing Liu, Liangjun Cheng, Huachao Li, Mei Ma
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引用次数: 0

Abstract

Objective: Acquired middle ear cholesteatomas (AMECs) is a pathological condition characterized by abnormal epithelial cell proliferation in the middle ear, often resulting from retraction pocket and epithelial migration theories. The aim of this study was to find the abnormal expressed circular RNAs (circ_RNAs) in AMECs and explore their role in the inflammatory response of AMECs.

Methods: Thirty-two AMECs patients and 32 healthy volunteers were recruited. Expression levels of circ_RNAs and inflammatory genes were quantified via reverse transcription quantitative polymerase chain reaction. Concentrations of inflammatory cytokines in human AMECs or normal post-auricular skin samples were measured using enzyme-linked immunosorbent assay.

Results: Results showed that circ_0006168 was higher expressed in AMECs. Receiver operating characteristic (ROC) curve analysis indicated that circ_0006168 might serve as a sensitive biomarker for diagnosing AMECs. Besides, AMECs samples showed increased inflammatory cytokines concentrations. Thirty-two AMECs patients were divided into the High (N = 17) and Low (N = 15) groups according to the expression of circ_0006168. The High group patients had more tinnitus and severe hearing loss than the Low group.

Conclusion: In summary, our findings suggested that circ_0006168 exacerbated inflammatory responses in AMECs, suggesting that circ_0006168 might be a potential biomarker in AMECs.

Circ_0006168表达可作为获得性中耳胆脂瘤的潜在生物标志物。
目的:获得性中耳胆脂瘤(AMECs)是一种以中耳上皮细胞异常增生为特征的病理状态,通常由回缩袋和上皮细胞迁移理论引起。本研究旨在发现AMECs中异常表达的环状rna (circ_RNAs),并探讨其在AMECs炎症反应中的作用。方法:招募32例AMECs患者和32名健康志愿者。通过逆转录定量聚合酶链反应定量检测circ_rna和炎症基因的表达水平。采用酶联免疫吸附法测定人AMECs或正常耳后皮肤样本中炎症细胞因子的浓度。结果:结果显示circ_0006168在AMECs中表达较高。受试者工作特征(ROC)曲线分析表明circ_0006168可作为诊断amec的敏感生物标志物。此外,AMECs样品显示炎症细胞因子浓度升高。根据circ_0006168的表达情况将32例AMECs患者分为High组(N = 17)和Low组(N = 15)。与低组相比,高组患者有更多的耳鸣和严重的听力损失。结论:综上所述,我们的研究结果表明circ_0006168加重了AMECs的炎症反应,表明circ_0006168可能是AMECs的潜在生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.30
自引率
7.70%
发文量
537
审稿时长
2-4 weeks
期刊介绍: Official Journal of European Union of Medical Specialists – ORL Section and Board Official Journal of Confederation of European Oto-Rhino-Laryngology Head and Neck Surgery "European Archives of Oto-Rhino-Laryngology" publishes original clinical reports and clinically relevant experimental studies, as well as short communications presenting new results of special interest. With peer review by a respected international editorial board and prompt English-language publication, the journal provides rapid dissemination of information by authors from around the world. This particular feature makes it the journal of choice for readers who want to be informed about the continuing state of the art concerning basic sciences and the diagnosis and management of diseases of the head and neck on an international level. European Archives of Oto-Rhino-Laryngology was founded in 1864 as "Archiv für Ohrenheilkunde" by A. von Tröltsch, A. Politzer and H. Schwartze.
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