Variation and morphological manifestation of germline restricted chromosomes in three species of leaf warblers of genus Phylloscopus.

IF 1.7 4区生物学 Q4 CELL BIOLOGY

Cytogenetic and Genome Research Pub Date : 2025-04-29 DOI:10.1159/000545903

Irina Ayvazyan, Ruzanna Petrosyan, Emma Khachatrian, Lianna Virabyan, Tigran Abgaryan, Zhenya Poghosyan, Yana Dombrovskaya, Marko Rakovic, Victor Spangenberg

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引用次数: 0

Abstract

Introduction: Germline restricted chromosomes are considered as a rather ancient element in the genomes of passerine birds. Obviously, detailed comparative studies on model groups including closely related species are required for a better understanding of GRC evolution. It is especially interesting if GRCs in such closely related species have acquired significant differences.

Methods: Analysis of synaptonemal complexes in spread nuclei of primary spermatocytes using immunocytochemical techniques and fluorescent microscopy were applied.

Results: Therefore, in this work we compared three taxonomically close species of the genus Phylloscopus: Ph. sindianus lorenzii, Ph. collybita menzbieri and Ph. nitidus, with differences in morphological manifestation of GRCs: macro- and micro-GRCs. We were able to trace the morphological transformations of macro-GRC at stages from leptotene to diplotene, and describe the complex structure of this chromosome in Ph. nitidus.

Conclusion: We hope that the taxonomic group under study can become a convenient model for comparative studies of GRCs in closely related species in order to understand the evolution of these unusual chromosomes.

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三种叶莺种系限制染色体的变异及形态表现。

种系限制性染色体被认为是雀形目鸟类基因组中一个相当古老的元素。显然，为了更好地理解GRC的进化，需要对包括近亲物种在内的模型群进行详细的比较研究。尤其有趣的是，在如此密切相关的物种中，GRCs是否获得了显著的差异。方法：应用免疫细胞化学技术和荧光显微镜对原代精母细胞弥散核突触复合体进行分析。结果：本研究比较了Phylloscopus属3个分类学相近的种：Ph. sindianus lorenzii, Ph. collybita menzbieri和Ph. nitidus，并比较了GRCs形态表现的差异：宏观和微观GRCs。我们能够追踪宏观grc在从瘦素到二倍体阶段的形态转变，并描述了该染色体在Ph. nitidus中的复杂结构。结论：我们希望所研究的分类类群能够成为近缘种GRCs比较研究的便捷模型，以了解这些异常染色体的进化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytogenetic and Genome Research 生物-细胞生物学

CiteScore

3.10

自引率

5.90%

发文量

审稿时长

1 months

期刊介绍： During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.