Design and Evaluation of Synthetic Microbial Communities for Effective Fire Blight Disease Control in Apples.

IF 3.1 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yejin Lee, Da-Ran Kim, Youn-Sig Kwak
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引用次数: 0

Abstract

Fire blight disease caused by Erwinia amylovora has resulted in extreme economic losses to industrial plants of the Rosaceae family, including apples and pears, since the 1870s. Many countries have used pesticides and bactericides to manage the fire blight disease. However, chemical management leads to the emergence of a pesticide-resistant pathogen population. Therefore, attempts at managing the fire blight disease have been developed and applied using selected microorganisms as a biological control. However, a single strain of the biological control agent showed limited effect in reducing the disease. Here, we designed synthetic microbial communities (SynCom), which involve reconstituting multi-strains rather than relying on a single strain to enhance the disease control efficiency. We constructed the SynCom based on three categorized functions: i) anti-EA strain, ii) keystone taxa in healthy apples, iii) abundant metabolite strain in healthy apples than the fire blight infected apples. SynCom was significantly efficient in suppressing fire blight, achieving 0% disease severity in apple fruits, 1.67% in roses, and 5.4% in apple plants. Our finding presented that the well-designed SynCom showed significant effects in controlling the fire blight disease. Additionally, SynCom members should be selected based on multiple functions to maximize the impact on crop management.

苹果火枯病防治综合微生物群落设计与评价。
自19世纪70年代以来,由葡萄霉引起的火疫病给蔷薇科的工业植物,包括苹果和梨,造成了极大的经济损失。许多国家已经使用杀虫剂和杀菌剂来控制火疫病。然而,化学品管理导致了抗药性病原体种群的出现。因此,在管理火疫病的尝试已经开发和应用,使用选定的微生物作为生物控制。然而,单一菌株的生物防治剂对减少该病的效果有限。在此,我们设计了合成微生物群落(SynCom),通过重组多菌株而不是依赖单一菌株来提高疾病控制效率。我们基于三个分类功能构建了SynCom: i)抗ea菌株,ii)健康苹果的关键类群,iii)健康苹果比火枯病感染的苹果代谢丰富的菌株。对苹果果实、玫瑰和苹果植株的严重程度分别为0%、1.67%和5.4%。研究结果表明,设计合理的SynCom对防治青枯病有显著的效果。此外,应根据多种功能选择SynCom成员,以最大限度地提高对作物管理的影响。
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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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