Description of a sheep abortion caused by atypical genotype of Toxoplasma gondii in the state of Mato Grosso, Brazil.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2025-04-25 DOI:10.1007/s11259-025-10745-5

Ícaro Guilherme Dos Santos, Marlon Ribeiro, João Paulo da Silva Cavasani, Luana Dias O Nascimento, Bernardo Nassau de Souza, Rafaela Mallmann-Bohn, Fábio Bernardo Schein, João Fabio Soares, Beatriz Souza Lima Nino, João Luis Garcia, Daniel Felipe Barrantes Murillo, Tatiane Terumi Negrão Watanabe, Daniel Moura de Aguiar, Caroline Argenta Pescador

{"title":"Description of a sheep abortion caused by atypical genotype of Toxoplasma gondii in the state of Mato Grosso, Brazil.","authors":"Ícaro Guilherme Dos Santos, Marlon Ribeiro, João Paulo da Silva Cavasani, Luana Dias O Nascimento, Bernardo Nassau de Souza, Rafaela Mallmann-Bohn, Fábio Bernardo Schein, João Fabio Soares, Beatriz Souza Lima Nino, João Luis Garcia, Daniel Felipe Barrantes Murillo, Tatiane Terumi Negrão Watanabe, Daniel Moura de Aguiar, Caroline Argenta Pescador","doi":"10.1007/s11259-025-10745-5","DOIUrl":null,"url":null,"abstract":"<p><p>Toxoplasma gondii is one of the major causes of abortion in sheep in many countries. We report an episode of a novel atypical genotype of T. gondii-associated abortion in a sheep flock belonging to the Veterinary School Hospital of the University of Cuiabá located in Midwestern Brazil. The group of animals was comprised of 15 adult sheep, and one abortion was detected in a gestating ewe. T. gondii indirect immunofluorescence assay performed in serum samples yielded an antibody titer of 1:8192 in the affected dam indicating an acute phase of infection. The aborted fetus and placenta were submitted for necropsy. The only significant gross findings were the placental cotyledons with white foci. Microscopic findings included multifocal necrotizing encephalitis with intralesional protozoal cysts in the fetus's brain and moderate multifocal lymphoplasmacytic necrotizing placentitis. Tissue samples of the fetus and placenta had a positive PCR result for T. gondii. Restriction fragment length polymorphism (RFLP) genotyping using markers (SAG1, 5'-SAG2, 3'-SAG2, Alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and APICO) was carried out and the results were consistent with an atypical genotype.</p>","PeriodicalId":23690,"journal":{"name":"Veterinary Research Communications","volume":"49 3","pages":"177"},"PeriodicalIF":1.8000,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Research Communications","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11259-025-10745-5","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Toxoplasma gondii is one of the major causes of abortion in sheep in many countries. We report an episode of a novel atypical genotype of T. gondii-associated abortion in a sheep flock belonging to the Veterinary School Hospital of the University of Cuiabá located in Midwestern Brazil. The group of animals was comprised of 15 adult sheep, and one abortion was detected in a gestating ewe. T. gondii indirect immunofluorescence assay performed in serum samples yielded an antibody titer of 1:8192 in the affected dam indicating an acute phase of infection. The aborted fetus and placenta were submitted for necropsy. The only significant gross findings were the placental cotyledons with white foci. Microscopic findings included multifocal necrotizing encephalitis with intralesional protozoal cysts in the fetus's brain and moderate multifocal lymphoplasmacytic necrotizing placentitis. Tissue samples of the fetus and placenta had a positive PCR result for T. gondii. Restriction fragment length polymorphism (RFLP) genotyping using markers (SAG1, 5'-SAG2, 3'-SAG2, Alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and APICO) was carried out and the results were consistent with an atypical genotype.

查看原文本刊更多论文

巴西马托格罗索州发生一起由非典型基因型刚地弓形虫引起的绵羊流产病例。

刚地弓形虫是许多国家绵羊流产的主要原因之一。我们报告了一个新的非典型基因型的弓形虫相关流产在羊群属于cuiabab大学兽医学校医院位于巴西中西部。这组动物由15只成年羊组成，在一只妊娠母羊中发现了一例流产。弓形虫间接免疫荧光测定在受影响的水坝的血清样本中显示抗体滴度为1:8192，表明感染处于急性期。流产胎儿及胎盘送检尸检。唯一显著的肉眼发现是胎盘子叶有白色的病灶。显微镜检查结果包括多灶性坏死性脑炎伴病灶内的胎儿脑原生动物囊肿和中度多灶性淋巴浆细胞坏死性胎盘炎。胎儿和胎盘组织标本PCR检测弓形虫阳性。采用标记（SAG1、5′-SAG2、3′-SAG2、Alt.SAG2、SAG3、BTUB、GRA6、c22-8、c29-2、L358、PK1和APICO）进行限制性内切片段长度多态性（RFLP）基因分型，结果与非典型基因型一致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.