Analysis of the association of influenza clinical course with single nucleotide polymorphisms in genes affecting the interferon-λ3 production.

Q3 Medicine
L I Nikolaeva, M D Stuchinskaya, K P Telepenina, N G Shevchenko, V V Kuprianov, K G Krasnoslobodtsev, E A Mukasheva, S V Trushakova, I N Khlopova, I S Kruzhkova, L B Kisteneva, L V Kolobukhina, E I Burtseva
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引用次数: 0

Abstract

Introduction: Predisposition to different courses of the infectious process is largely associated with the polymorphisms in human genome, especially in genes encoding proteins of the immune system. In the early stages of influenza infection such components of innate immunity as interferons I (α/β) and III (λ) type play a significant role in limiting virus replication. The aim of the work was to investigate associations of single nucleotide polymorphism in IFNL3 (rs8099917 T/G) and IFNL4 (rs12979860 C/T) genes with different course of influenza, and identify genetic markers of influenza complicated by community-acquired pneumonia. The genes noted above affect the production of interferon-λ3, which is involved in restriction of the viral replication.

Materials and methods: Samples from 456 patients with mild (n = 150), moderate (n = 173), and severe (n = 133) influenza were studied. The viral RNA was detected by reverse transcription and polymerase chain reaction (RT-PCR). Polymorphisms in IFNL3 (rs8099917 T/G) and IFNL4 (rs12979860 C/T) genes was detected by PCR. Statistical analysis was performed using SNPStats software.

Results: Patients with the C/T or T/T genotype of IFNL4 gene (rs12979860 C/T) were more likely to have pneumonia than those with the C/C genotype (OR 2.47 (1.31-4.63); p = 0.0044; q = 0.0059). The presence of one T allele increased the risk of developing pneumonia (OR 2.02 (1.05-4.02); p = 0.006; q = 0.008). In the presence of the T/T genotype, the risk increased more than twofold: OR 2.14 (1.31-3.48). Analysis of the SNP of IFNL3 gene (rs8099917 T/G) revealed a weak association of the G allele with pneumonia (OR 1.86 (1.04-3.31); p = 0.03; q = 0.045).

Conclusion: Genetic markers of increased risk of community-acquired pneumonia in influenza include the presence of the T allele in IFNL4 gene (rs12979860 C/T) and, to a lesser extent, the G allele in IFNL3 gene (rs8099917 T/G). Patients carrying these alleles have an increased risk of developing pneumonia, especially in old age.

影响干扰素-λ3产生的基因单核苷酸多态性与流感临床病程的关系分析
导读:对不同感染过程的易感性在很大程度上与人类基因组的多态性有关,特别是在编码免疫系统蛋白质的基因中。在流感感染的早期阶段,诸如干扰素I (α/β)和干扰素III (λ)型等先天免疫成分在限制病毒复制方面发挥重要作用。本研究旨在探讨IFNL3 (rs8099917 T/G)和IFNL4 (rs12979860 C/T)基因单核苷酸多态性与不同流感病程的相关性,并鉴定流感合并社区获得性肺炎的遗传标记。上述基因影响干扰素-λ3的产生,而干扰素-λ3参与限制病毒复制。材料与方法:对456例轻度(n = 150)、中度(n = 173)和重度(n = 133)流感患者的样本进行研究。采用逆转录和聚合酶链反应(RT-PCR)检测病毒RNA。PCR检测IFNL3 (rs8099917 T/G)和IFNL4 (rs12979860 C/T)基因多态性。采用SNPStats软件进行统计分析。结果:IFNL4基因C/T或T/T基因型(rs12979860 C/T)患者比C/C基因型患者更易发生肺炎(or为2.47 (1.31-4.63);P = 0.0044;Q = 0.0059)。存在1个T等位基因会增加患肺炎的风险(OR 2.02 (1.05-4.02);P = 0.006;Q = 0.008)。存在T/T基因型时,风险增加了两倍以上:OR为2.14(1.31-3.48)。对IFNL3基因(rs8099917 T/G)的SNP分析显示,G等位基因与肺炎存在弱相关性(OR 1.86 (1.04-3.31);P = 0.03;Q = 0.045)。结论:流感患者社区获得性肺炎风险增加的遗传标记包括IFNL4基因中T等位基因(rs12979860 C/T)的存在,以及IFNL3基因中G等位基因(rs8099917 T/G)的存在。携带这些等位基因的患者患肺炎的风险增加,尤其是在老年人中。
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来源期刊
Voprosy virusologii
Voprosy virusologii Medicine-Infectious Diseases
CiteScore
2.00
自引率
0.00%
发文量
48
期刊介绍: The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.
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