Deep phenotyping of human neutrophils in whole blood using a 33-color spectral flow cytometry panel.

IF 3.6 3区医学 Q3 CELL BIOLOGY

Journal of Leukocyte Biology Pub Date : 2025-05-07 DOI:10.1093/jleuko/qiaf049

Vanessa Krémer, Marion Rambault, Sandrine Schmutz, Nicolas Montcuquet, Pierre Bruhns, Luc de Chaisemartin, Friederike Jönsson

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引用次数: 0

Abstract

Neutrophils are the most abundant leukocytes in the circulation and critical players in host defense and inflammation. They respond rapidly to numerous biological, chemical, and physical stimuli, making it challenging to characterize their steady-state phenotypes, activation states, and subsets in an unbiased and precise manner. To address this problem, we designed a 33-color spectral flow cytometry panel for the deep profiling of unprocessed neutrophils in human blood. This panel allows the profiling of neutrophil phenotypes related to activation, immune modulation, granule release, ontogeny, phagocytic capacity, and migration, in addition to monitoring all major human leukocyte populations. We validated the panel using whole blood stimulations that induce distinct phenotypic shifts in the neutrophil population. This optimized spectral flow cytometry panel allows comprehensive immune profiling of the functional heterogeneity of human blood neutrophils and is suitable for longitudinal or exploratory analysis of neutrophil dynamics and activation states in clinical cohorts.

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利用33色光谱流式细胞仪对人全血中性粒细胞进行深度表型分析。

中性粒细胞是循环中最丰富的白细胞，在宿主防御和炎症中起着关键作用。它们对许多生物、化学和物理刺激反应迅速，这使得以公正和精确的方式表征它们的稳态表型、激活状态和亚群具有挑战性。为了解决这个问题，我们设计了一个33色光谱流式细胞仪面板，用于人类血液中未处理的中性粒细胞的深度分析。除了监测所有主要的人类白细胞群外，该小组还可以分析与活化、免疫调节、颗粒释放、个体发生、吞噬能力和迁移相关的中性粒细胞表型。我们使用全血刺激来验证该小组，该刺激在中性粒细胞群体中诱导明显的表型变化。这种优化的光谱流式细胞仪面板允许对人类血液中性粒细胞的功能异质性进行全面的免疫分析，适用于临床队列中中性粒细胞动力学和激活状态的纵向或探索性分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Leukocyte Biology 医学-免疫学

CiteScore

11.50

自引率

0.00%

发文量

358

审稿时长

2 months

期刊介绍： JLB is a peer-reviewed, academic journal published by the Society for Leukocyte Biology for its members and the community of immunobiologists. The journal publishes papers devoted to the exploration of the cellular and molecular biology of granulocytes, mononuclear phagocytes, lymphocytes, NK cells, and other cells involved in host physiology and defense/resistance against disease. Since all cells in the body can directly or indirectly contribute to the maintenance of the integrity of the organism and restoration of homeostasis through repair, JLB also considers articles involving epithelial, endothelial, fibroblastic, neural, and other somatic cell types participating in host defense. Studies covering pathophysiology, cell development, differentiation and trafficking; fundamental, translational and clinical immunology, inflammation, extracellular mediators and effector molecules; receptors, signal transduction and genes are considered relevant. Research articles and reviews that provide a novel understanding in any of these fields are given priority as well as technical advances related to leukocyte research methods.