High Prevalence of Enterocytozoon bieneusi Genotype BEB6 in Wild Boars in Lorestan Province, Iran: Potential Source of Zoonotic Transmission.

IF 0.9 4区医学 Q4 PARASITOLOGY

Iranian Journal of Parasitology Pub Date : 2025-01-01 DOI:10.18502/ijpa.v20i1.18113

Ehsan Javanmard, Hanieh Mohammad Rahimi, Ali Taghipour, Sara Nemati, Mehdi Mohebali, Mostafa Rezaeian, Ali Badrifar, Elham Kazemirad, Hamed Mirjalali

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引用次数: 0

Abstract

Background: We aimed to investigate the prevalence of Enterocytozoon bieneusi and the circulating genotypes in wild boar in the western regions of Iran.

Methods: Fifty-two fecal samples were collected from wild boars in Lorestan province, Iran. After DNA extraction, the presence of E. bieneusi was evaluated by real-time PCR. A nested PCR targeting the internal transcribed region (ITS) was employed to characterize genotypes. The PCR products were sequenced, and the genetic diversity and relationships among the genotypes were identified using MEGA X and DnaSp (V5) software.

Results: Sixteen (30%) samples were positive for E. bieneusi using real-time PCR, and 11 (21%) were positive for nested PCR, which was sequenced. All 11 positive samples were identified as the BEB6 genotype (Group 2). The haplotype diversity was noted to be 0.182, and the nucleotide diversity, calculated using DnaSp, was 0. 00085.

Conclusion: The findings highlight the high prevalence of the genotype BEB6 in wild boars. The presence of this genotype suggests the circulation of E. bieneusi between domesticated animals and wild boars in Iran.

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BEB6基因型在伊朗洛雷斯坦省野猪中高发：人畜共患病传播的潜在来源

背景：本研究旨在调查伊朗西部地区野猪中布氏肠胞虫的流行情况和流行基因型。方法：采集伊朗洛雷斯坦省52例野猪粪便标本。提取DNA后，采用实时荧光定量PCR法检测是否存在比氏伊氏杆菌。采用巢式PCR技术对其内部转录区（ITS）进行基因型鉴定。对PCR产物进行测序，利用MEGA X和DnaSp （V5）软件鉴定基因型间的遗传多样性和相互关系。结果：16份标本实时荧光定量PCR阳性（30%），11份标本巢式荧光定量PCR阳性（21%）。11份阳性样本均鉴定为BEB6基因型（第二组）。单倍型多样性为0.182，核苷酸多样性为0。00085.结论：BEB6基因型在野猪中具有较高的流行率。该基因型的存在表明，在伊朗，家养动物和野猪之间存在比氏伊氏杆菌的传播。

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来源期刊

Iranian Journal of Parasitology PARASITOLOGY-

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.