Validating the antidiabetic potential of Nakima (Tupistra clarkei Hook.f.), a traditional food from eastern Himalayan region, through network pharmacology and in vivo experimentation.

IF 2.8 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Sutapa Datta, Soumita Bhattacharjee, Supriyo Ghosh, Amlan Jyoti Ghosh, Tilak Saha, Arnab Sen
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引用次数: 0

Abstract

Objective: To explore and understand the antidiabetic activity of Tupistra clarkei Hook.f. inflorescence, providing a scientific explanation to the ethnomedicinal properties.

Methods: The constituents of the plant were determined through GC-MS analysis, which were used for target prediction and network pharmacology to understand how the plant regulates hyperglycaemia and other diabetes complications. These properties were validated in vivo along with further assessment of the antioxidant potential of the plant, both in vitro and in vivo.

Key findings: The plant showed good phenol-flavonoid content, and antioxidant potential both in vitro and in vivo. GC-MS analysis identified 24 constituents of the plant. In silico analysis showed their ability to target 166 proteins that are associated with pathways in controlling hyperglycaemia and other diabetic consequences, protection of pancreatic tissue, insulin secretion, and insulin resistance. This was reflected in the in vivo experiment where T. clarkei showed ability to reduce FBG, LDL-C, VLDL-C levels, improve the levels of HDL-C, and also facilitate reversal of damage in pancreatic islets.

Conclusion: Our study validated the antidiabetic potential Tupistra clarkei inflorescence in the in silico and in vivo assessment, and has proved to have good antioxidant activity and potential against diabetes. However, further clinical trials are essential.

通过网络药理学和体内实验验证喜马拉雅东部地区传统食物Nakima (Tupistra clarkei Hook.f.)的抗糖尿病潜力。
目的:探讨和了解兔皮草的降糖作用。花序,为其民族医药特性提供了科学的解释。方法:采用气相色谱-质谱法测定其成分,用于靶点预测和网络药理学,了解其对高血糖等糖尿病并发症的调节作用。这些特性在体内得到验证,并进一步评估了植物在体外和体内的抗氧化潜力。主要发现:该植物具有良好的酚类黄酮含量,体外和体内均具有抗氧化潜力。GC-MS分析鉴定出该植物的24种成分。计算机分析显示,它们能够靶向166种蛋白质,这些蛋白质与控制高血糖和其他糖尿病后果、保护胰腺组织、胰岛素分泌和胰岛素抵抗的途径有关。这在体内实验中得到了体现,克拉氏t细胞显示出降低FBG、LDL-C、VLDL-C水平的能力,提高HDL-C水平,并促进胰岛损伤的逆转。结论:本研究通过体外和体内实验验证了大花楸花序的抗糖尿病潜能,并证明其具有良好的抗氧化活性和抗糖尿病潜能。然而,进一步的临床试验是必要的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.60
自引率
0.00%
发文量
91
审稿时长
3 months
期刊介绍: JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.
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