Deciphering the heterogeneous glucosinolates composition in leaves and seeds: strategies for developing Brassica napus genotypes with low seed glucosinolates content but high leaf glucosinolates content.

Abstract

Rapeseed cakes with low glucosinolates content (GC) possess high feeding value. However, the pursuit of low-GC seeds has inadvertently resulted in a reduction of GC in leaves, making plants more susceptible to stress and lowering their nutritional quality. Therefore, it is imperative to disrupt the tight association between GC in these two tissues and ultimately develop genotypes with low-GC seeds but high-GC leaves. The distinct mechanisms underlying glucosinolate (GSL) synthesis in these two tissues remain unclear. Here, we discovered that aliphatic and aromatic GSLs, rather than indole GSLs, contribute to the positive correlation between GC in seeds and leaves. We performed selective-sweep analyses and identified the genomic footprints left after decades of intense selection for low-GC seeds. By conducting genome-wide association studies and analyzing differentially expressed genes in high- and low-GC seeds and leaves, we compiled lists of distinct genes involved in GSL synthesis in leaves and seeds separately. In particular, BnMYB28 plays a key role in regulating GC in both seeds and leaves. Selection and manipulation of BnaC09.MYB28 would affect GC in both tissues. However, downregulation of BnaA02.MYB28 and/or BnaC02.MYB28 would likely reduce GC in seeds without causing a concurrent reduction in GC in leaves.