Association mapping and identification of candidate genes for callus induction and regeneration using sorghum mature seeds.

IF 4.1 2区生物学 Q1 PLANT SCIENCES

Frontiers in Plant Science Pub Date : 2025-04-24 eCollection Date: 2025-01-01 DOI:10.3389/fpls.2025.1430141

Jingyi Xu, Lihua Wang, Yuan Liang, Qi Shen, Wenmiao Tu, Zhengxiao Cheng, Lu Hu, Yi-Hong Wang, Jieqin Li

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引用次数: 0

Abstract

Introduction: A whole plant can be regenerated through tissue culture from an embryogenic callus in a process referred to as plant regeneration. Regeneration ability of embryogenic callus is a quantitative trait and the main limiting factor for genetic studies in sorghum.

Methods: We evaluated 236 sorghum mini core varieties for callus induction rate, embryogenic callus rate, callus browning rate and differentiation rate and performed a multi-locus genome-wide association study (GWAS) of the four traits with 6,094,317 SNPs.

Results: We found five mini core varieties most amenable to tissue culture manipulations: IS5667, IS24503, IS8348, IS4698, and IS5295.Furthermore, we mapped 34 quantitative trait loci (QTLs) to the four traits and identified 47 candidate genes. Previous studies provided evidence for the orthologs of 14 of these genes for their role in cellular function and embryogenesis and that the ortholog of WIND1 (WOUND INDUCED DEDIFFERENTIATION 1) identified in this study promotes callus formation and increases de novo shoot regeneration.

Conclusion: These candidate genes will help to further understand the genetic basis of plant embryonic callus regeneration.

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高粱成熟种子愈伤组织诱导与再生候选基因的关联定位与鉴定。

整个植株可以通过胚性愈伤组织培养再生，这一过程被称为植株再生。高粱胚性愈伤组织再生能力是一个数量性状，也是遗传研究的主要限制因素。方法：对236个高粱小核心品种的愈伤组织诱导率、胚性愈伤组织率、愈伤组织褐化率和分化率进行了评价，并对这4个性状进行了多位点全基因组关联研究（GWAS）。结果：我们找到了5个最适合组织培养的迷你核心品种：IS5667、IS24503、IS8348、IS4698和IS5295。此外，我们将34个数量性状位点（qtl）定位到4个性状上，并鉴定出47个候选基因。先前的研究已经证实了这些基因中的14个同源基因在细胞功能和胚胎发生中的作用，并且本研究中发现的WIND1 （WOUND INDUCED DEDIFFERENTIATION 1）同源基因促进愈伤组织形成并增加新生芽再生。结论：这些候选基因有助于进一步了解植物胚愈伤组织再生的遗传基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Plant Science PLANT SCIENCES-

CiteScore

7.30

自引率

14.30%

发文量

4844

审稿时长

14 weeks

期刊介绍： In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches. Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.