Identifying the Alkaline Tolerance-Related Genes Through Transcriptome Analysis of Halomonas alkalicola CICC 11012 s.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-05-12 DOI:10.1007/s00284-025-04178-1

Lei Zhai, Shuaicheng Mu, Ruina Liu, Rui Liu, Geer Lin, Qi Han, Su Yao

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Abstract

Halomonas alkalicola CICC 11012 s is the strongest alkaliphile in the genus Halomonas. So far, studies have focused on the genome level and functional validation of a single gene, providing an overview and partial analysis of the adaptive mechanisms. As such, the comprehensive adaptations of alkaliphiles to extremely alkaline stress remain largely unclear. Therefore, in this study, the transcriptome profiling of H. alkalicola under neutral and alkaline conditions was compared to explore its global adaptation mechanisms towards pH homeostasis. In addition, the different up-regulated genes of this strain grown at pH 11.0 were compared with those grown at pH 7.0. The results revealed that the up-regulated genes were mainly distributed in six categories, including glycosyl transferase, fimbrial assembly protein, TonB-dependent transport system, C₄-dicarboxylate TRAP transport system, transposase, and toxin-antitoxin system. This result indicated that H. alkalicola developed various adaptive strategies to survive under extremely alkaline pressure, from modifying their cell wall structure to enhancing their membrane transport activities and intracellular metabolism homeostasis. Furthermore, the function of the gene cluster tonB-exbB-exbB2-exbD under extreme alkaline stress was verified by the CRISPR-Cas9 gene-editing system, indicating that the TonB-dependent transport system significantly affected the growth of the strain under extreme alkaline stresses.

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通过对碱单胞菌ccic 11012s的转录组分析鉴定碱耐受性相关基因。

Halomonas alkicola CICC 11012s是盐单胞菌属中最强的亲碱菌。到目前为止，研究主要集中在基因组水平和单个基因的功能验证上，对其适应机制进行了概述和部分分析。因此，嗜碱菌对极碱性胁迫的全面适应在很大程度上仍不清楚。因此，本研究比较了H. alkicola在中性和碱性条件下的转录组谱，探讨其对pH稳态的全球适应机制。此外，还比较了该菌株在pH 11.0和pH 7.0条件下的不同上调基因。结果表明，上调基因主要分布在糖基转移酶、毛膜组装蛋白、tonb依赖转运系统、c4 -二羧酸TRAP转运系统、转座酶和毒素-抗毒素系统等6类。这一结果表明，从改变细胞壁结构到增强膜转运活性和细胞内代谢稳态，碱杆菌发展了多种适应策略来适应极端碱性压力下的生存。此外，通过CRISPR-Cas9基因编辑系统验证了tonB-exbB-exbB2-exbD基因簇在极端碱性胁迫下的功能，表明tonb依赖性转运系统显著影响了菌株在极端碱性胁迫下的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.