CD44 and EPCAM expression in pleomorphic adenoma and mucoepidermoid carcinoma: An immunohistochemical method.

Q2 Dentistry
Dental Research Journal Pub Date : 2025-04-24 eCollection Date: 2025-01-01 DOI:10.4103/drj.drj_545_24
Laleh Maleki, Bahareh Fattahi, Seyed Mohammad Razavi, Mina Shekarian, Shahnaz Eskandari
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引用次数: 0

Abstract

Background: Recent studies have indicated that assessing the expression levels of cancer stem cell markers is critical in predicting the behavior of these neoplasms. This study aimed to evaluate and compare the expression levels of CD44 and epithelial cell adhesion molecule (EpCAM) markers in pleomorphic adenoma (PA) and mucoepidermoid carcinoma (MEC) using immunohistochemistry.

Materials and methods: In this cross-sectional descriptive-analytical study, 20 samples each of PA and MEC were selected from Kashani Hospital, Isfahan, Iran, based on inclusion and exclusion criteria. Specimens were prepared using immunohistochemical methods and analyzed under an optical microscope. Pathologists evaluated microscopic grade, staining intensity and percentage, and the staining intensity distribution (SID) index. Statistical analysis was conducted with SPSS (version 26), employing the Kolmogorov-Smirnov test, t-test, Chi-square, and Fisher's exact test.

Results: The mean frequency of stained cells for both CD44 (P = 0.39) and EpCAM (P = 0.40) markers showed no statistically significant differences between the PA and MEC groups. Similarly, the mean intensity of staining did not differ significantly for either CD44 (P = 0.40) or EpCAM (P = 0.18). The average SID index for the EpCAM marker in the MEC group was significantly higher than the PA group (P = 0.03) and for the EpCAM marker, there was a significant difference between the average SID index and all three variables of microscopic grade (P = 0.01), clinical stage (P = 0.00), and 3-year prognosis (P = 0.02).

Conclusion: The use of EpCAM immunohistochemical marker may help to predict the behavior of salivary gland tumors and obtain better treatment measures for patients.

CD44和EPCAM在多形性腺瘤和黏液表皮样癌中的表达:免疫组织化学方法。
背景:最近的研究表明,评估肿瘤干细胞标志物的表达水平对预测这些肿瘤的行为至关重要。本研究旨在应用免疫组织化学方法评价和比较多形性腺瘤(PA)和黏液表皮样癌(MEC)中CD44和上皮细胞粘附分子(EpCAM)标志物的表达水平。材料和方法:在这项横断面描述性分析研究中,根据纳入和排除标准,从伊朗伊斯法罕Kashani医院选择PA和MEC各20例样本。采用免疫组织化学方法制备标本,在光学显微镜下进行分析。病理学家评估显微分级、染色强度和百分比以及染色强度分布(SID)指数。统计分析采用SPSS (version 26)软件,采用Kolmogorov-Smirnov检验、t检验、卡方检验和Fisher确切检验。结果:PA组和MEC组CD44 (P = 0.39)和EpCAM (P = 0.40)标记的染色细胞平均频率差异无统计学意义。同样,CD44 (P = 0.40)和EpCAM (P = 0.18)的平均染色强度也没有显著差异。MEC组EpCAM标记物的平均SID指数显著高于PA组(P = 0.03), EpCAM标记物的平均SID指数与显微分级(P = 0.01)、临床分期(P = 0.00)、3年预后(P = 0.02)三个变量的差异均有统计学意义。结论:EpCAM免疫组化标志物的应用有助于预测唾液腺肿瘤的行为,为患者提供更好的治疗措施。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Dental Research Journal
Dental Research Journal Dentistry-Dentistry (all)
CiteScore
1.70
自引率
0.00%
发文量
70
审稿时长
52 weeks
期刊介绍: Dental Research Journal, a publication of Isfahan University of Medical Sciences, is a peer-reviewed online journal with Bimonthly print on demand compilation of issues published. The journal’s full text is available online at http://www.drjjournal.net. The journal allows free access (Open Access) to its contents and permits authors to self-archive final accepted version of the articles on any OAI-compliant institutional / subject-based repository. The journal will cover technical and clinical studies related to health, ethical and social issues in field of Dentistry. Articles with clinical interest and implications will be given preference.
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