Spot-14 and its paralog Spot-14R regulate expression of metabolic and thermogenic pathway genes in murine brown and beige adipocytes

IF 3.5 4区生物学 Q1 Biochemistry, Genetics and Molecular Biology

FEBS Letters Pub Date : 2025-05-02 DOI:10.1002/1873-3468.70052

Lidia Itzel Castro-Rodríguez, Cristina Velez-delValle, Claudia Patricia Hernández-Mosqueira, Walid Kuri-Harcuch

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引用次数: 0

Abstract

Spot 14 (S14), encoded by Thrsp, is a thyroid hormone-responsive transcriptional activator that regulates lipogenesis, though its mechanisms remain unclear. We aimed to study the role of S14 on gene expression in adipocytes. We analyzed Thrsp and its paralog Mid1ip1 in brown (EB5), beige (EB7), and white (F442A) adipocytes. Thrsp expression was higher in EB5 and EB7 than in F442A and increased with thyroid hormone T3 in EB5 and EB7 but decreased in F442A. Mid1ip1 expression rose moderately in EB5 and EB7, influencing lipid metabolism genes. Silencing Thrsp upregulated Mid1ip1 in EB7 and reduced thermogenic gene expression in EB5 and EB7. These findings underscore the roles of Thrsp and Mid1ip1 in metabolic and thermogenic pathways, highlighting the responsiveness of S14 to thyroid hormones and nutrient signals.

Impact statement

This study reveals that Thyroid Hormone-Induced Protein 8 (THRSP), also known as Spot-14, and its paralog Spot-14R, regulate metabolic and thermogenic gene expression differently in brown and beige adipocytes. These findings provide insights into adipocyte metabolism, offering potential targets for obesity and metabolic disorder treatments.

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Spot-14及其类似物Spot-14R调节小鼠棕色和米色脂肪细胞的代谢和产热途径基因的表达。

由Thrsp编码的斑点14 （S14）是一种甲状腺激素应答转录激活因子，可调节脂肪生成，但其机制尚不清楚。我们旨在研究S14在脂肪细胞基因表达中的作用。我们分析了Thrsp及其在棕色（EB5）、米色（EB7）和白色（F442A）脂肪细胞中的类似Mid1ip1。Thrsp在EB5和EB7中的表达高于F442A，并且随着甲状腺激素T3的增加，在EB5和EB7中表达增加，而在F442A中表达减少。在EB5和EB7中，Mid1ip1表达适度升高，影响脂质代谢基因。沉默Thrsp可上调EB7中的Mid1ip1，降低EB5和EB7中的产热基因表达。这些发现强调了Thrsp和Mid1ip1在代谢和产热途径中的作用，强调了S14对甲状腺激素和营养信号的响应性。本研究表明，甲状腺激素诱导蛋白8 (THRSP)，也称为Spot-14，及其类似的Spot-14R，在棕色和米色脂肪细胞中调节代谢和产热基因表达的方式不同。这些发现提供了对脂肪细胞代谢的见解，为肥胖和代谢紊乱的治疗提供了潜在的靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

FEBS Letters 生物-生化与分子生物学

CiteScore

7.00

自引率

2.90%

发文量

303

审稿时长

1.0 months

期刊介绍： FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.