Association of Glutathione S-transferase gene polymorphism with coronary artery disease (CAD) in North Indian population (Jammu and Kashmir): evidence from a case-control study and an updated meta-analysis.
Jyotdeep K Raina, Ravi Sharma, Naveen Kumar, Sheikh Abid Ali, Rakesh K Panjaliya, Ashok Bakaya, Parvinder Kumar
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引用次数: 0
Abstract
Background: Chemicals released during cigarette smoking disrupt the structure, function and physiological capacity of the cardiovascular system. Detoxification of these harmful chemicals is done by Glutathione S-transferase (GST) isoenzymes (GSTM1 and GSTT1). GST gene polymorphisms may have a role in conferring susceptibility to coronary artery disease. This case-control study aims to evaluate the relationship between GSTM1 and GSTT1 gene polymorphisms, smoking habits, and coronary artery disease (CAD) in the Northern Indian population of Jammu and Kashmir, strengthened by a meta-analysis based on previously published studies.
Methods: The current study involved 220 patients with CAD and 240 healthy controls from the Jammu region in the Union Territory of Jammu and Kashmir. Whole blood DNA was isolated, followed by genotyping using the polymerase chain reaction (PCR) technique.
Results: Smoking, a non-vegetarian diet, and lipid levels were found to be significantly associated with coronary artery disease (CAD). The frequency of the GSTMnull genotype was significantly higher in patients than in controls (48.2% vs. 33.3%), while both groups showed comparable frequencies of the GSTTnull genotype. Combined genotype analysis indicated that the GSTM1 Tnull genotype was associated with an increased risk of CAD, with an adjusted odds ratio (AOR) of 1.70 and a 95% confidence interval (CI) of 1.30-2.27(p = 0.05). Patients who were smokers and had the GSTMnull genotype, as well as those with the GSTM1Tnull or GSTMnullT1 genotypes, were at a significantly higher risk of developing CAD. The results of the meta-analysis supported the findings of the case-control association study.
Conclusion: The GSTM1 null genotype, either independently or in conjunction with smoking, is linked to the incidence of CAD among North Indians in Jammu and Kashmir.
期刊介绍：
Inhalation Toxicology is a peer-reviewed publication providing a key forum for the latest accomplishments and advancements in concepts, approaches, and procedures presently being used to evaluate the health risk associated with airborne chemicals.
The journal publishes original research, reviews, symposia, and workshop topics involving the respiratory system’s functions in health and disease, the pathogenesis and mechanism of injury, the extrapolation of animal data to humans, the effects of inhaled substances on extra-pulmonary systems, as well as reliable and innovative models for predicting human disease.
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