Rhamnocitrin induces apoptosis of human nasopharyngeal carcinoma by inhibiting IGF-1R signaling pathway in vitro.

IF 1.3 3区医学 Q3 CHEMISTRY, APPLIED

Journal of Asian Natural Products Research Pub Date : 2025-04-21 DOI:10.1080/10286020.2025.2491611

Yan-Yuan Zhou, Ya-Zhou Li, Zhi-Qiang Liu, Xiao-Hui Qin, Ya-Qi Wu, Chao-Jie Chong, Liang Lyu, Cheng-Xue Pan

引用次数: 0

Abstract

This study explored rhamnocitrin's apoptotic effects on human nasopharyngeal carcinoma cell lines (CNE-2/C666-1) and underlying mechanisms. Following treatment with varying concentrations, cell proliferation, apoptosis, and protein expression were analyzed using MTT assay, Hoechst/Annexin V-FITC/PI staining, and western blot. Results showed rhamnocitrin inhibited cell proliferation, induced apoptosis, downregulated IGF-1R, Erk1/2, and Akt phosphorylation, and activated caspase 3, caspase 8, caspase 9, and Bax while inhibiting survivin, Bcl-2, and Mcl-1. In conclusion, rhamnocitrin induces apoptosis in nasopharyngeal carcinoma cells by inhibiting the IGF-1R signaling pathway and its downstream effectors Akt and Erk1/2.

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鼠李柠檬素通过抑制IGF-1R信号通路诱导鼻咽癌细胞凋亡。

本研究探讨鼠李柠檬素对人鼻咽癌细胞株（CNE-2/C666-1）凋亡的影响及其机制。不同浓度处理后，采用MTT法、Hoechst/Annexin V-FITC/PI染色和western blot分析细胞增殖、凋亡和蛋白表达。结果表明，鼠李柑苷抑制细胞增殖，诱导细胞凋亡，下调IGF-1R、Erk1/2和Akt磷酸化，激活caspase 3、caspase 8、caspase 9和Bax，抑制survivin、Bcl-2和Mcl-1。综上所述，鼠李柑苷通过抑制IGF-1R信号通路及其下游效应物Akt和Erk1/2诱导鼻咽癌细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Asian Natural Products Research 医学-药学

CiteScore

3.20

自引率

5.90%

发文量

审稿时长

2.3 months

期刊介绍： The Journal of Asian Natural Products Research (JANPR) publishes chemical and pharmaceutical studies in the English language in the field of natural product research on Asian ethnic medicine. The journal publishes work from scientists in Asian countries, e.g. China, Japan, Korea and India, including contributions from other countries concerning natural products of Asia. The journal is chemistry-orientated. Major fields covered are: isolation and structural elucidation of natural constituents (including those for non-medical uses), synthesis and transformation (including biosynthesis and biotransformation) of natural products, pharmacognosy, and allied topics. Biological evaluation of crude extracts are acceptable only as supporting data for pure isolates with well-characterized structures. All published research articles in this journal have undergone rigorous peer review, based on initial editor screening and anonymized refereeing by at least two expert referees.