Induction of Fetal Hydrocephalus Followed by Ventriculoamniotic Shunting and End of Study Analysis in the Pregnant Sheep Model.

IF 1.6 3区医学 Q3 OBSTETRICS & GYNECOLOGY

Fetal Diagnosis and Therapy Pub Date : 2025-05-03 DOI:10.1159/000546161

Stephen Paul Emery, Stephanie Greene, Nika Marie Hazen, Youngjae Chun

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引用次数: 0

Abstract

Introduction: As part of an evidence-based reassessment of ventriculoamniotic shunting for fetal aqueductal stenosis, we developed an animal model for the induction of hydrocephalus, placement of a ventriculoamniotic shunt, and end of study analysis in fetal sheep.

Methods: We made multiple revisions to both the animal model and the shunt over time. Regarding the animal model, we found that induction of hydrocephalus at 85 days gestation, shunt placement at 100 days gestation, and study end at 135 days gestation generated the most reproducible results.

Conclusion: The purpose of this manuscript was to document step-by-step instructions for hydrocephalus induction, shunt placement, and end of study analysis so that those who wish to develop a fetal hydrocephalus model, repeat our experience with ventriculoamniotic shunting, or test other interventions may benefit from our experience. Moreover, standardization of methodology will allow for meaningful comparisons between investigators.

查看原文本刊更多论文

妊娠羊模型诱导胎儿脑积水并发脑室-羊膜分流及研究结束分析。

引言：作为对脑室-羊膜分流治疗胎儿输水管狭窄的循证再评估的一部分，我们建立了一个动物模型，用于诱导脑积水，放置脑室-羊膜分流，并在胎羊中进行研究结束分析。方法：随着时间的推移，我们对动物模型和分流器进行了多次修改。在动物模型中，我们发现妊娠85天诱导脑积水、妊娠100天放置分流器、妊娠135天结束研究的结果可重复性最好。结论：本文的目的是记录脑积水诱导、分流放置和研究结束分析的逐步说明，以便那些希望建立胎儿脑积水模型、重复我们的脑室-羊膜分流经验或测试其他干预措施的人可以从我们的经验中受益。此外，方法的标准化将允许研究者之间进行有意义的比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fetal Diagnosis and Therapy 医学-妇产科学

CiteScore

4.70

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： The first journal to focus on the fetus as a patient, ''Fetal Diagnosis and Therapy'' provides a wide range of biomedical specialists with a single source of reports encompassing the common discipline of fetal medicine.