In-Situ Synthesis of Silver Nanoparticle within Self-Assembling Ultrashort Peptide Hydrogel as Antibacterial with Wound Healing Properties.

IF 1.9 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Firuza Begum, Aman Kumar Mahto, Shalini Kumari, Rikeshwer Prasad Dewangan
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引用次数: 0

Abstract

Introduction/objectives: Silver nanoparticles [AgNPs] are promising antimicrobial agents, but their synthesis often involves toxic reducing agents. To address this, we developed a green synthesis methodology employing an in-situ approach for synthesizing AgNPs within self- -assembled ultrashort peptide hydrogels through photochemical synthesis, eliminating the need for toxic chemicals.

Methods: A novel tetrapeptide was designed and synthesized to form hydrogels in aqueous solutions. AgNPs were incorporated into the hydrogel via in-situ photochemical synthesis using sunlight. The hydrogel and AgNPs were characterized through spectroscopic and microscopic techniques. The antibacterial efficacy of the AgNP-loaded hydrogel was assessed against gram-positive and gram-negative bacteria, and its wound-healing potential in mammalian cell lines was evaluated.

Results: Among the peptides synthesized, PHG-2 formed a hydrogel at a 1% w/v concentration in aqueous solution. Characterization using the gel inversion assay, circular dichroism [CD] spectroscopy, and transmission electron microscopy [TEM] revealed uniform nanofibril self-assembly. UV spectroscopy and TEM confirmed the formation of AgNPs within the hydrogel. While the peptide hydrogel exhibited moderate antibacterial activity alone, the AgNP-loaded hydrogel demonstrated synergistic antibacterial effects against methicillin-resistant Staphylococcus aureus [MRSA] and Escherichia coli. A docking study of all the synthesized peptides was performed against FmtA [an enzyme for cell wall synthesis of MRSA] and results were correlated with the obtained docking score. The silver-loaded peptide hydrogel showed a twofold increase in antibacterial activity against MRSA compared to silver nitrate solutions. The hydrogel significantly promoted wound healing in HEK-293T and MCF-7 cells compared to the control.

Conclusions: This study introduces a novel ultrashort tetrapeptide sequence for developing antibacterial agents that are effective against infected wounds while supporting wound healing. Utilizing in-situ photochemical synthesis, the green synthesis approach provides an environmentally friendly and sustainable alternative to conventional methods.

自组装超短肽水凝胶中银纳米粒子的原位合成及其抗菌创面愈合性能。
银纳米颗粒[AgNPs]是很有前途的抗菌剂,但它们的合成通常涉及毒性还原剂。为了解决这个问题,我们开发了一种绿色合成方法,采用原位方法通过光化学合成在自组装的超短肽水凝胶中合成AgNPs,从而消除了对有毒化学物质的需求。方法:设计并合成一种新型四肽,使其在水溶液中形成水凝胶。AgNPs通过利用太阳光进行原位光化学合成而被纳入水凝胶中。通过光谱学和显微技术对水凝胶和AgNPs进行了表征。研究了负载agnp的水凝胶对革兰氏阳性菌和革兰氏阴性菌的抗菌效果,并对其在哺乳动物细胞系中的伤口愈合潜力进行了评价。结果:在所合成的肽中,PHG-2以1% w/v的浓度在水溶液中形成水凝胶。利用凝胶倒置实验、圆二色光谱和透射电子显微镜进行表征,发现纳米纤维具有均匀的自组装。紫外光谱和透射电镜证实了水凝胶中AgNPs的形成。虽然肽水凝胶单独表现出中等的抗菌活性,但负载agnp的水凝胶对耐甲氧西林金黄色葡萄球菌[MRSA]和大肠杆菌表现出协同抗菌作用。将所有合成的肽与FmtA (MRSA细胞壁合成酶)进行对接研究,结果与获得的对接评分相关。与硝酸银溶液相比,载银肽水凝胶对MRSA的抗菌活性增加了两倍。与对照组相比,水凝胶显著促进HEK-293T和MCF-7细胞的伤口愈合。结论:本研究引入了一种新的超短四肽序列,用于开发抗感染创面并支持创面愈合的抗菌药物。利用原位光化学合成,绿色合成方法提供了一种环境友好和可持续的替代传统方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current protein & peptide science
Current protein & peptide science 生物-生化与分子生物学
CiteScore
5.20
自引率
0.00%
发文量
73
审稿时长
6 months
期刊介绍: Current Protein & Peptide Science publishes full-length/mini review articles on specific aspects involving proteins, peptides, and interactions between the enzymes, the binding interactions of hormones and their receptors; the properties of transcription factors and other molecules that regulate gene expression; the reactions leading to the immune response; the process of signal transduction; the structure and function of proteins involved in the cytoskeleton and molecular motors; the properties of membrane channels and transporters; and the generation and storage of metabolic energy. In addition, reviews of experimental studies of protein folding and design are given special emphasis. Manuscripts submitted to Current Protein and Peptide Science should cover a field by discussing research from the leading laboratories in a field and should pose questions for future studies. Original papers, research articles and letter articles/short communications are not considered for publication in Current Protein & Peptide Science.
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