D Dhyan Chowdary, Y Sridhar, G Ramachandra Rao, N Anusha, S M Rahman, B Sravanthi, S K Mangrauthia, G K Srividya
{"title":"Fitness cost and molecular basis of imidacloprid resistance in brown planthopper, Nilaparvata lugens (Stål) in India.","authors":"D Dhyan Chowdary, Y Sridhar, G Ramachandra Rao, N Anusha, S M Rahman, B Sravanthi, S K Mangrauthia, G K Srividya","doi":"10.1007/s10646-025-02894-9","DOIUrl":null,"url":null,"abstract":"<p><p>This study aims to assess the fitness cost associated with imidacloprid resistance and expression of cytochrome P450 genes in brown planthopper, Nilaparvata lugens (Stål). After continuous selection with imidacloprid exposure over 10 generations in a glass house, N. lugens developed a 12.84-fold resistance. The demographic and life history parameters of imidacloprid resistant (IMI-R) and susceptible (IMI-S) strains of N. lugens were compared by age-stage, two-sex life table approach. The duration of egg, third instar, fifth instar, pre-adult and total pre-oviposition period were significantly prolonged in IMI-R strain. However, the fecundity of IMI-R strain (194.59) was significantly lower as compared to IMI-S strain (224.05). There was no significant effect on the duration of other nymphal instars and adult longevity. Whereas the intrinsic rate of increase and doubling time differed significantly in the IMI-R and IMI-S strains, the relative fitness of IMI-R strain was 0.74, clearly indicating a trade-off between imidacloprid resistance and fitness in N. lugens. In IMI-R strain, two P450 genes CYP6ER1 and CYP6AY1 were significantly upregulated by 5.85 and 3.35-fold, respectively compared to the IMI-S strain. Our results conclude that imidacloprid resistance in N. lugens has significant fitness cost due to prolonged developmental stages, reduced fecundity, altered demographic parameters and upregulation of P450 genes. Lower fitness of imidacloprid resistant strains has direct implication in management of N. lugens in rice ecosystems, as any withdrawal of exposure could potentially recover susceptibility.</p>","PeriodicalId":11497,"journal":{"name":"Ecotoxicology","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ecotoxicology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1007/s10646-025-02894-9","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This study aims to assess the fitness cost associated with imidacloprid resistance and expression of cytochrome P450 genes in brown planthopper, Nilaparvata lugens (Stål). After continuous selection with imidacloprid exposure over 10 generations in a glass house, N. lugens developed a 12.84-fold resistance. The demographic and life history parameters of imidacloprid resistant (IMI-R) and susceptible (IMI-S) strains of N. lugens were compared by age-stage, two-sex life table approach. The duration of egg, third instar, fifth instar, pre-adult and total pre-oviposition period were significantly prolonged in IMI-R strain. However, the fecundity of IMI-R strain (194.59) was significantly lower as compared to IMI-S strain (224.05). There was no significant effect on the duration of other nymphal instars and adult longevity. Whereas the intrinsic rate of increase and doubling time differed significantly in the IMI-R and IMI-S strains, the relative fitness of IMI-R strain was 0.74, clearly indicating a trade-off between imidacloprid resistance and fitness in N. lugens. In IMI-R strain, two P450 genes CYP6ER1 and CYP6AY1 were significantly upregulated by 5.85 and 3.35-fold, respectively compared to the IMI-S strain. Our results conclude that imidacloprid resistance in N. lugens has significant fitness cost due to prolonged developmental stages, reduced fecundity, altered demographic parameters and upregulation of P450 genes. Lower fitness of imidacloprid resistant strains has direct implication in management of N. lugens in rice ecosystems, as any withdrawal of exposure could potentially recover susceptibility.
期刊介绍：
Ecotoxicology is an international journal devoted to the publication of fundamental research on the effects of toxic chemicals on populations, communities and terrestrial, freshwater and marine ecosystems. It aims to elucidate mechanisms and processes whereby chemicals exert their effects on ecosystems and the impact caused at the population or community level. The journal is not biased with respect to taxon or biome, and papers that indicate possible new approaches to regulation and control of toxic chemicals and those aiding in formulating ways of conserving threatened species are particularly welcome. Studies on individuals should demonstrate linkage to population effects in clear and quantitative ways. Laboratory studies must show a clear linkage to specific field situations. The journal includes not only original research papers but technical notes and review articles, both invited and submitted. A strong, broadly based editorial board ensures as wide an international coverage as possible.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
