Proposal of a new chemical marker for the quality control of the herb Scleromitrion diffusum.

IF 3.8 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Frontiers in Chemistry Pub Date : 2025-04-29 eCollection Date: 2025-01-01 DOI:10.3389/fchem.2025.1600769

Tao Zheng, Chu-Chu Zhong, Grace Gar-Lee Yue, Dong-Min Jiang, Man-Ho Tong, Hiu-Yan Wong, Kwan-Ho Wong, Meng-Hua Wu, David Tai-Wai Lau, Hui Cao, Pang-Chui Shaw, Clara Bik-San Lau

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引用次数: 0

Abstract

Baihuasheshecao (Scleromitrion diffusum (Willd.) R.J. Wang), a traditional Chinese medicinal herb, is extensively used as an ingredient in herbal teas and is a well-known folk medicine in China. However, two common adulterants, Hedyotis corymbosa (L.) Lam. and Hedyotis tenelliflora (BL.) Kuntze, belonging to the same family, are frequently mistaken as S. diffusum in folk medicine. This confusion has led to a growing concern about their identification and quality evaluation. While previous studies have demonstrated over 15 various compounds as potential chemical markers for authentication and quality control for S. diffusum, considering the selection criteria for chemical markers in the Chinese Pharmacopoeia, most of these compounds do not meet the pharmacopoeial requirements. Hence, this study aims to identify an appropriate chemical marker for S. diffusum based on thin layer chromatography (TLC) identification and high-performance liquid chromatography (HPLC) quality control, in order to differentiate from any counterfeits such as H. corymbosa and H. tenelliflora. Our findings suggested for the first time that the compound (E)-6-O-(p-coumaroyl) scandoside methyl ester which is specific to S. diffusum, could serve as the chemical marker. Here, attempt was made to establish standardized protocols for TLC identification, HPLC analysis, and quantitative methodologies, adhering to Chinese pharmacopoeial standards, along with laboratory scale preparation procedures. In conclusion, our findings have provided valuable insights to the identification and quality control processes for S. diffusum, and anticipated with potential applications by the industry and regulatory authority.

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一种新的化学标记物用于药材弥散硬皮的质量控制。

白花佘藻（野生白花佘藻）王r.j.)是一种传统的中草药，广泛用于草药茶的成分，是中国著名的民间药物。然而，两种常见的掺假剂，蛇舌草（L.）林。与蛇舌草同属一科，在民间医学中常被误认为是白花蛇舌草。这种混淆导致了对它们的识别和质量评估的日益关注。虽然已有15种以上的化合物可作为弥散草鉴别和质量控制的潜在化学标记物，但考虑到中国药典中化学标记物的选择标准，这些化合物大多不符合药典要求。因此，本研究旨在通过薄层色谱（TLC）鉴别和高效液相色谱（HPLC）质量控制，建立合适的弥散香化学标记，以鉴别假药如山茱萸（H. corymbosa）和天冬花（H. tenelliflora）。本研究结果首次表明，扩散葡萄球菌特有的化合物(E)-6- o -（对香豆醇基）甘露苷甲酯可作为化学标记物。在此，试图建立标准化的薄层色谱鉴别、高效液相色谱分析和定量方法方案，遵循中国药典标准，以及实验室规模制备程序。本研究为弥散香的鉴别和质量控制提供了有价值的见解，并为弥散香的行业和监管部门提供了潜在的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Chemistry Chemistry-General Chemistry

CiteScore

8.50

自引率

3.60%

发文量

1540

审稿时长

12 weeks

期刊介绍： Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide. Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”. All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.