The effect of in vitro use of FSH on sperm parameters, DNA integrity, and mitochondrial membrane potential in asthenozoospermic men.

IF 1.9 4区医学 Q3 UROLOGY & NEPHROLOGY

International Urology and Nephrology Pub Date : 2025-11-01 Epub Date: 2025-05-08 DOI:10.1007/s11255-025-04540-z

Faezeh Etebari, Mohammad Ebrahim Rezvani, Sahar Khosravi, Mahin Izadi, Esmat Mangoli

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引用次数: 0

Abstract

Sperm motility is a key indicator of male fertility. Decreased motility, or asthenozoospermia, highlights the need for understanding male fertility challenges. This study was designed to evaluate the effects of in vitro use of follicle-stimulating hormone (FSH) on various sperm parameters, sperm DNA integrity, and mitochondrial membrane potential. Semen samples were obtained from 20 asthenozoospermic men. Each sample was divided into control and case groups. In the case group, samples were incubated with FSH at 30 mIU/mL for 1 h, while in the control group, the samples were incubated for 1 h without treatment. Sperm parameters, DNA fragmentation, and mitochondrial membrane potential were assessed in the two groups based on WHO 2021 criteria. Progressive motility and rapid progressive motility were higher in the case group compared to the control. However, FSH did not have a significant effect on morphology, viability, DNA fragmentation, or mitochondrial membrane potential in either group. In conclusion, FSH effectively enhanced sperm motility without compromising sperm DNA integrity or mitochondrial membrane potential (MMP). Therefore, FSH can be recommended as a safe and effective option for sperm selection in patients with asthenozoospermia.

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体外使用卵泡刺激素对精子参数、DNA完整性和线粒体膜电位的影响。

精子活力是男性生育能力的关键指标。运动能力下降，或弱精子症，强调需要了解男性生育能力的挑战。本研究旨在评估体外使用促卵泡激素（FSH）对各种精子参数、精子DNA完整性和线粒体膜电位的影响。采集了20例弱精子症患者的精液样本。每个样本被分为对照组和病例组。病例组用30 mIU/mL的FSH孵育1 h，对照组不加处理孵育1 h。根据WHO 2021标准对两组的精子参数、DNA断裂和线粒体膜电位进行评估。与对照组相比，病例组的进行性运动和快速进行性运动更高。然而，FSH对两组小鼠的形态学、活力、DNA断裂或线粒体膜电位均无显著影响。综上所述，FSH在不影响精子DNA完整性或线粒体膜电位（MMP）的情况下有效地增强了精子活力。因此，FSH可作为一种安全有效的精子选择方法推荐给弱精子症患者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Urology and Nephrology 医学-泌尿学与肾脏学

CiteScore

3.40

自引率

5.00%

发文量

329

审稿时长

1.7 months

期刊介绍： International Urology and Nephrology publishes original papers on a broad range of topics in urology, nephrology and andrology. The journal integrates papers originating from clinical practice.