{"title":"The upregulation of caveolin-1 expression via the TLR4-Myd88 pathway contributes to cerebral vasospasm.","authors":"Quan Yan, Tai'an Fang, Xuefei Liu, Guojia Du","doi":"10.1080/01616412.2025.2491778","DOIUrl":null,"url":null,"abstract":"<p><p>Cerebral vasospasm (CVS) following subarachnoid hemorrhage (SAH) significantly impacts patient morbidity and mortality. This study aimed to investigate the role of the TLR4-MyD88 signaling pathway in the pathogenesis of CVS and its relationship with Caveolin-1 (CAV-1). We established a rat model of SAH using the double blood injection method in the cisterna magna to induce CVS. Various techniques, including pathological assessments, immunohistochemistry, Western blotting, and RT-qPCR, were employed to evaluate the expression of Toll-like receptor 4 (TLR4), MyD88, NF-κB, and CAV-1. The results showed a significant upregulation of TLR4, MyD88, NF-κB, and CAV-1 in SAH rats, with the upregulation correlating with CVS. Furthermore, the application of methyl-β-cyclodextrin (MβCD) effectively reduced CAV-1 expression and ameliorated CVS severity. Additionally, dexmedetomidine (DEX) treatment downregulated inflammatory markers and improved CVS, suggesting a potential therapeutic role through modulating the TLR4-MyD88 pathway. This study highlights the complex interplay between neuroinflammation, CAV-1, and CVS, proposing new avenues for therapeutic interventions in managing CVS post-SAH.</p>","PeriodicalId":19131,"journal":{"name":"Neurological Research","volume":" ","pages":"594-603"},"PeriodicalIF":1.5000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurological Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/01616412.2025.2491778","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/22 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CLINICAL NEUROLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Cerebral vasospasm (CVS) following subarachnoid hemorrhage (SAH) significantly impacts patient morbidity and mortality. This study aimed to investigate the role of the TLR4-MyD88 signaling pathway in the pathogenesis of CVS and its relationship with Caveolin-1 (CAV-1). We established a rat model of SAH using the double blood injection method in the cisterna magna to induce CVS. Various techniques, including pathological assessments, immunohistochemistry, Western blotting, and RT-qPCR, were employed to evaluate the expression of Toll-like receptor 4 (TLR4), MyD88, NF-κB, and CAV-1. The results showed a significant upregulation of TLR4, MyD88, NF-κB, and CAV-1 in SAH rats, with the upregulation correlating with CVS. Furthermore, the application of methyl-β-cyclodextrin (MβCD) effectively reduced CAV-1 expression and ameliorated CVS severity. Additionally, dexmedetomidine (DEX) treatment downregulated inflammatory markers and improved CVS, suggesting a potential therapeutic role through modulating the TLR4-MyD88 pathway. This study highlights the complex interplay between neuroinflammation, CAV-1, and CVS, proposing new avenues for therapeutic interventions in managing CVS post-SAH.
期刊介绍:
Neurological Research is an international, peer-reviewed journal for reporting both basic and clinical research in the fields of neurosurgery, neurology, neuroengineering and neurosciences. It provides a medium for those who recognize the wider implications of their work and who wish to be informed of the relevant experience of others in related and more distant fields.
The scope of the journal includes:
•Stem cell applications
•Molecular neuroscience
•Neuropharmacology
•Neuroradiology
•Neurochemistry
•Biomathematical models
•Endovascular neurosurgery
•Innovation in neurosurgery.