Molecular assessment of Coxiella burnetii in horses in Northwestern Iran.

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2025-04-01 DOI:10.18502/ijm.v17i2.18389

Somayyeh Hosseinzadeh, Katayoon Nofouzi, Faezah Hasanzadeh, Saber Esmaeili, Esmail Ayen

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Abstract

Background and objectives: Q fever is a frequently occurring illness that is induced by the bacterium Coxiella burnetii (C. burnetii) that can infect humans and various animals. It targets the macrophage cells in the tissues, and circulating monocytes.

Materials and methods: This study was conducted between 2022 and 2023 in the West Azerbaijan and Ardabil provinces of northwestern Iran to examine the presence infection of C. burnetii. Specimens were obtained by swabbing from 140 mares (70 from each province) and 20 stallions (10 from each province) which were apparently healthy, and their DNA was analyzed using quantitative PCR assay detecting the IS1111 element of the bacterium.

Results: The findings indicated that a mere 0.625% of the examined specimens tested positive for C. burnetii. Among the entire set of specimens, a single female horse from the region of Ardabil was found to be the carrier of the bacterium.

Conclusion: This suggested that even though horses may not display any clinical symptoms, they can still harbor C. burnetii and contribute to its transmission. Therefore, the potential contribution of horses to Q fever transmission should be considered.

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伊朗西北部马科希氏杆菌的分子鉴定。

背景和目的：Q热是一种由伯纳氏杆菌（C. burnetii）引起的常见病，可感染人类和各种动物。它的目标是组织中的巨噬细胞和循环中的单核细胞。材料和方法：本研究于2022年至2023年在伊朗西北部的西阿塞拜疆和阿达比尔省进行，以检查伯纳蒂胞杆菌感染的存在。选取140匹健康母马（各省70匹）和20匹健康公马（各省10匹），采用定量PCR法检测细菌IS1111元素，对其DNA进行分析。结果：检测结果表明，伯纳蒂胞杆菌阳性检出率仅为0.625%。在整套标本中，来自阿达比尔地区的一匹母马被发现是这种细菌的携带者。结论：这表明，即使马可能没有表现出任何临床症状，它们仍然可以携带伯氏c并促进其传播。因此，应考虑马对Q热传播的潜在贡献。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.