High-Throughput Metabolomic Profiling of Skin Lesions: Comparative Study of Cutaneous Squamous Cell Carcinoma, Basal Cell Carcinoma, and Normal Skin Via e-Biopsy Sampling.

IF 2.3 4区医学 Q3 BIOPHYSICS

Cellular and molecular bioengineering Pub Date : 2025-04-03 eCollection Date: 2025-04-01 DOI:10.1007/s12195-025-00846-1

Leetal Louie, Julia Wise, Ariel Berl, Ofir Shir-Az, Vladimir Kravtsov, Zohar Yakhini, Avshalom Shalom, Alexander Golberg, Edward Vitkin

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引用次数: 0

Abstract

Purpose: Rising rates of cutaneous squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC) make standard histopathology diagnostic methods a bottleneck. Using tissue molecular information for diagnostics offers a promising alternative. Faster specimen collection and high-throughput molecular identification can improve the processing of the increasing number of tumors. This study aims (i) to confirm the ability of e-biopsy technique to harvest metabolites, (ii) to obtain high-resolution metabolomic profiles of cSCC, BCC, and healthy skin tissues, and (iii) to perform a comparative analysis of the collected profiles.

Methods: Tumor specimens were collected with electroporation-based biopsy (e-biopsy), a minimally invasive sampling collection tool, from 13 tissue samples (cSCC, BCC, and healthy skin) from 12 patients. Ultra performance liquid chromatography and tandem mass spectrometry (UPLC-MS-MS) was used for molecular identification and quantification of resulting metabolomic profiles.

Results: Here we report measurements of 2325 small metabolites identified (301 with high confidence) in 13 tissue samples from 12 patients. Comparative analysis identified 34 significantly (p < 0.05) differentially expressed high-confidence metabolites. Generally, we observed a greater number of metabolites with higher expression, in cSCC and in BCC compared to healthy tissues, belonging to the subclass amino acids, peptides, and analogues.

Conclusions: These findings confirm the ability of e-biopsy technique to obtain high-resolution metabolomic profiles suitable to downstream bioinformatics analysis. This highlights the potential of e-biopsy coupled with UPLC-MS-MS for rapid, high-throughput metabolomic profiling in skin cancers and supports its utility as a promising diagnostic alternative to standard histopathology.

Supplementary information: The online version contains supplementary material available at 10.1007/s12195-025-00846-1.

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皮肤病变的高通量代谢组学分析：通过电子活检取样对皮肤鳞状细胞癌、基底细胞癌和正常皮肤的比较研究。

目的：皮肤鳞状细胞癌（cSCC）和基底细胞癌（BCC）发病率的上升使标准的组织病理学诊断方法成为瓶颈。利用组织分子信息进行诊断提供了一个很有前途的选择。更快的标本采集和高通量分子鉴定可以改善对越来越多的肿瘤的处理。本研究旨在(i)确认电子活检技术收集代谢物的能力，（ii）获得cSCC、BCC和健康皮肤组织的高分辨率代谢组学图谱，以及（iii）对收集到的图谱进行比较分析。方法：采用电穿孔活检（e-biopsy）这一微创取样工具，从12例患者的13个组织样本（cSCC、BCC和健康皮肤）中收集肿瘤标本。采用超高效液相色谱-串联质谱（UPLC-MS-MS）对代谢组学图谱进行分子鉴定和定量。结果：在这里，我们报告了从12名患者的13个组织样本中鉴定出的2325种小代谢物（301种具有高置信度）的测量结果。结论：这些发现证实了电子活检技术获得适合下游生物信息学分析的高分辨率代谢组学图谱的能力。这突出了电子活检结合UPLC-MS-MS在皮肤癌中快速、高通量代谢组学分析的潜力，并支持其作为标准组织病理学诊断替代方案的应用前景。补充信息：在线版本包含补充资料，可在10.1007/s12195-025-00846-1获取。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cellular and molecular bioengineering 生物-生物物理

CiteScore

5.60

自引率

3.60%

发文量

审稿时长

>12 weeks

期刊介绍： The field of cellular and molecular bioengineering seeks to understand, so that we may ultimately control, the mechanical, chemical, and electrical processes of the cell. A key challenge in improving human health is to understand how cellular behavior arises from molecular-level interactions. CMBE, an official journal of the Biomedical Engineering Society, publishes original research and review papers in the following seven general areas: Molecular: DNA-protein/RNA-protein interactions, protein folding and function, protein-protein and receptor-ligand interactions, lipids, polysaccharides, molecular motors, and the biophysics of macromolecules that function as therapeutics or engineered matrices, for example. Cellular: Studies of how cells sense physicochemical events surrounding and within cells, and how cells transduce these events into biological responses. Specific cell processes of interest include cell growth, differentiation, migration, signal transduction, protein secretion and transport, gene expression and regulation, and cell-matrix interactions. Mechanobiology: The mechanical properties of cells and biomolecules, cellular/molecular force generation and adhesion, the response of cells to their mechanical microenvironment, and mechanotransduction in response to various physical forces such as fluid shear stress. Nanomedicine: The engineering of nanoparticles for advanced drug delivery and molecular imaging applications, with particular focus on the interaction of such particles with living cells. Also, the application of nanostructured materials to control the behavior of cells and biomolecules.