Limited impact of the siRNA pathway on transposable element expression in Aedes aegypti.

Abstract

Background: Transposable elements (TEs) are DNA sequences that can change their position within a genome. In insects, small RNA pathways are central to the transcriptional and post-transcriptional regulation of TE expression. The Piwi-interacting RNA (piRNA) pathway is particularly important in germline tissues, where it silences TE transcripts via small RNAs of 24-30 nucleotides (nt) in length produced from genomic precursor transcripts as well as through a "ping-pong" amplification cycle. The small interfering RNA (siRNA) pathway helps restrict TE expression in somatic tissues via 21nt small RNAs produced from double-stranded RNA by the endonuclease Dicer2, which guide an RNA-induced silencing complex to degrade complementary RNAs. However, much of this knowledge comes from studies of the model insect Drosophila melanogaster. In the mosquito Aedes aegypti, a medically significant vector species, the siRNA pathway has mainly been investigated in connection with its antiviral role, leaving open whether it also regulates TE expression.

Results: We investigated the expression of TEs and small RNAs in both somatic and gonadal tissues of a Dicer2 mutant line of Ae. aegypti and its wild-type counterpart. Our results show a modified pattern of TE expression and a decrease in TE-derived 21nt RNAs in the Dicer2 mutant, but no major shift of TE transcript abundance. The lack of a functional siRNA pathway also causes perturbations in piRNA ping-pong signatures and the expression of certain piRNA-associated genes, but without clear evidence for compensation by increased piRNA pathway activity.

Conclusions: The mosquito Ae. aegypti produces siRNAs derived from TEs but these lack a critical role in the regulation of TE expression both in somatic and in gonadal tissues.