N terminus and C terminus of Arabidopsis P4-ATPase AtALA1 facilitate the detoxification of the mycotoxin deoxynivalenol in wheat.

IF 7.5 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2025-05-05 DOI:10.1016/j.celrep.2025.115641

Fanlong Wang, Mingliang Qiu, Lei Hou, Xianbi Li, Hui Ren, Jingxin Zhuo, Haoru Liu, Yujie Li, Yang Yang, Xingying Yan, Mi Zhang, Dan Jin, Ting Lan, Jianyan Zeng, Yanhua Fan, Yang Yuan, Zhengqiang Ma, Yan Pei

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引用次数: 0

Abstract

Fusarium head blight (FHB) is a devastating disease affecting many important cereal crops. The disease is mainly caused by Fusarium graminearum, which produces mycotoxins, e.g., deoxynivalenol (DON), that contaminate grains, leading to serious issues in food safety worldwide. Here, we demonstrate that expressing the Arabidopsis P4-ATPase flippase gene, AtALA1, significantly increases wheat resistance to FHB, while substantially reducing DON content in grains. However, expressing TaALA1s, the wheat homologs of AtALA1, does not enhance the resistance. We discovered that the N terminus and C terminus of AtALA1 are crucial for its role in DON detoxification. The motif [DE]_nX_1-2FXX[FL]XXXR, found exclusively in the N terminus of ALA1s in Brassicaceae species, facilitates AtALA1-associated vesicle transport by binding with AP-2. Meanwhile the DON-stimulated phosphorylation at three key sites in the C terminus is responsible for promoting DON trafficking into vacuoles. Our findings suggest a great potential for vesicle-associated detoxification in FHB resistance and food safety.

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拟南芥P4-ATPase AtALA1的N端和C端促进小麦真菌毒素脱氧雪腐镰刀菌醇的解毒。

赤霉病（Fusarium head blight， FHB）是一种影响许多重要谷类作物的毁灭性病害。该疾病主要由谷物镰刀菌引起，它产生真菌毒素，如脱氧雪腐镰刀菌醇（DON），污染谷物，在世界范围内导致严重的食品安全问题。本研究表明，表达拟南芥p4 - atp酶翻转酶基因AtALA1可以显著提高小麦对FHB的抗性，同时显著降低籽粒中DON的含量。然而，表达AtALA1的小麦同源物TaALA1s并没有增强抗性。我们发现AtALA1的N端和C端对其解毒DON的作用至关重要。[DE]nX1-2FXX[FL]XXXR基序仅存在于十字花科植物ala1的N端，通过与AP-2结合促进atala1相关的囊泡运输。同时，在C末端的三个关键位点，DON刺激的磷酸化负责促进DON运输到液泡中。我们的研究结果表明，在FHB抗性和食品安全方面，囊泡相关解毒具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.