A V Sentyabreva, M A Diatroptova, E A Miroshnichenko, A D Gerasimov, A M Kosyreva
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引用次数: 0
Abstract
Inflammaging is one of the main risk factors for the development and progression of age-related diseases. The increase in the proinflammatory background and ROS production can lead to persistent activation and dysfunction of microglial cells. The biomolecular and functional changes in microglial cells after long-term exposure to prooxidant aluminum chloride were studied in in vitro experiment. BV2 cells were cultured in the presence of 0.5 and 1 mM AlCl3 for 70 or 140 h. The intensity of ROS production, apoptosis, and M1/M2 phenotype polarization of microglia were assessed by flow cytometry, qPCR-RT, and ELISA. Cells cultured with 0.5 mM AlCl3 showed signs of "healthy aging", while the higher concentration (1 mM) of AlCl3 led to persistent activation of microglia. The data obtained can be used for in vitro modeling of inflammaging and related physiological and pathological processes.
炎症是年龄相关疾病发生和发展的主要危险因素之一。促炎背景和ROS产生的增加可导致小胶质细胞的持续激活和功能障碍。通过体外实验研究了长期暴露于促氧化剂氯化铝后小胶质细胞的生物分子和功能变化。将BV2细胞分别在0.5和1 mM AlCl3中培养70或140 h。采用流式细胞术、qPCR-RT和ELISA检测小胶质细胞的ROS产生强度、凋亡强度和M1/M2表型极化。0.5 mM AlCl3培养的细胞表现出“健康衰老”的迹象，而较高浓度（1 mM）的AlCl3则导致小胶质细胞的持续活化。所获得的数据可用于体外模拟炎症及相关的生理和病理过程。
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
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