The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristem.

IF 7.5 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2025-05-06 DOI:10.1016/j.celrep.2025.115660

Fatimah Aljedaani, Yinghui Luo, Yanming Deng, Wouter Smet, Zeeshan Nasim, Xinjing Xu, Umar F Shahul Hameed, Ting Ting Xiao, Jose Kenyi Gonzalez-Kise, Stefan Arold, Ikram Blilou

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引用次数: 0

Abstract

In the root meristem of Arabidopsis, stem cell maintenance depends on the coordinated action of transcription factor networks. The transcriptional regulator EMBRYO DEFECTIVE 1579 (EMB1579), a protein that forms nuclear condensates, regulates plant growth. However, the molecular mechanisms through which it functions in the root meristem remain largely unclear. Here, we show that EMB1579 is required for stem cell maintenance and proper cell division orientation. EMB1579 modulates the function of two root stem cell regulatory modules, PLETHORAs and SCARECROW-SHORT-ROOT, through a process involving transcriptional regulation and RNA splicing. We show that EMB1579 acts as a catalyst for stem cell gene expression, and its activity is fine-tuned by its physical association with RNA splicing factors. The formation of nuclear condensates is essential for EMB1579 function in the root meristem. Our findings reveal a mechanism by which EMB1579 regulates stem cell determinants in the root meristem and expand the understanding of gene regulation complexity in plant development.

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EMB1579在转录和剪接中的双重功能支配着拟南芥根分生组织的组织模式。

在拟南芥根分生组织中，干细胞的维持依赖于转录因子网络的协同作用。转录调节剂胚胎缺陷1579 （EMB1579）是一种形成核凝聚体的蛋白质，调节植物生长。然而，它在根分生组织中起作用的分子机制仍不清楚。在这里，我们证明了EMB1579是干细胞维持和适当的细胞分裂方向所必需的。EMB1579通过涉及转录调控和RNA剪接的过程调节两个根干细胞调控模块PLETHORAs和稻草人- short - root的功能。我们发现EMB1579作为干细胞基因表达的催化剂，其活性通过与RNA剪接因子的物理关联而得到微调。核凝聚体的形成是EMB1579在根分生组织中发挥作用所必需的。我们的发现揭示了EMB1579调控根分生组织中干细胞决定因素的机制，并扩大了对植物发育中基因调控复杂性的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.