Construction of Corynebacterium glutamicum chassis for preferential utilization of xylose by adaptive laboratory evolution.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology Letters Pub Date : 2025-05-05 DOI:10.1007/s10529-025-03588-2

Mingxin Gao, Xi Sun, Yiyang Liu, Tao Chen, Zhiwen Wang

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引用次数: 0

Abstract

Objectives: To fully utilize lignocellulosic hydrolysate abundant in xylose content, it is necessary to engineer a Corynebacterium glutamicum strain that can preferentially and efficiently utilize xylose in the presence of glucose/xylose mixtures.

Results: C. glutamicum strain CGS15X5-E2 was obtained through metabolic engineering and adaptive laboratory evolution (ALE), which preferentially utilizes xylose and completely consumes it before switching to glucose utilization. A genetically defined chassis strain, CGS15X57, was constructed to switch to glucose consumption only after xylose was depleted based on genome analysis and mutation reconstruction, in which xylose utilization capability was also enhanced. The average xylose consumption rate of CGS15X57 reached 0.833 ± 0.048 g/l/h, which was 28.0% higher than that of the control. Three new beneficial mutations (Cgl1992^{267 insert}, Cgl2948^{G208 T} and Cgl2948^556△C) endow C. glutamicum with rapid growth and efficient xylose utilization phenotypes.

Conclusions: A chassis strain of C. glutamicum that preferentially and efficiently utilizes xylose has been obtained, facilitating the full utilization of lignocellulosic hydrolysates and the construction of co-culture systems under glucose/xylose mixed sugar conditions.

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谷氨酸棒状杆菌优先利用木糖基质的实验室适应性进化构建。

目的：为了充分利用富含木糖的木质纤维素水解产物，有必要设计一种在葡萄糖/木糖混合物存在下能优先高效利用木糖的谷氨酸棒状杆菌菌株。结果：通过代谢工程和适应性实验室进化（ALE）获得谷氨酰胺C. glutamum菌株CGS15X5-E2，该菌株优先利用木糖，在完全消耗木糖后才转向葡萄糖利用。通过基因组分析和突变重建，构建了一个基因定义的底盘菌株CGS15X57，该菌株在木糖耗尽后才转向葡萄糖消耗，木糖利用能力也得到了增强。CGS15X57的平均木糖消耗率为0.833±0.048 g/l/h，比对照提高28.0%。3个新的有益突变（Cgl1992267 insert、Cgl2948G208 T和Cgl2948556△C）使谷氨酰胺具有快速生长和高效利用木糖的表型。结论：获得了一株优先高效利用木糖的谷氨酰胺基底菌株，为充分利用木质纤维素水解产物和构建葡萄糖/木糖混合糖条件下的共培养体系提供了条件。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.