{"title":"Polymicrobial detection and salivary metabolomics of children with early childhood caries.","authors":"Ting Pan, YuJia Ren, JingYi Li, Ying Liao, XiangHui Xing","doi":"10.7717/peerj.19399","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Early childhood caries (ECC) has been proposed to be associated with various microorganisms and metabolites. This study aims to compare the prevalence of specific microbial species and salivary metabolomics profile in children with and without ECC, and to explore the correlation between salivary metabolites and targeted microbes.</p><p><strong>Method: </strong>Five ml of unstimulated saliva was collected from 32 ECC and 22 caries-free children. Clinical indexed were recorded and questionnaires regarding oral health and dietary habits were obtained from the guardians. The presence of eight specific microbial species were examined using species-specific quantitative PCR (qPCR). Untargeted metabolomics was analyzed to identify key differential metabolites and pathways. Correlations among clinical, microbial, and metabolomic data were further explored.</p><p><strong>Results: </strong>The prevalence of <i>Scardovia wiggsiae</i> (90.6%, <i>P</i> < 0.001), <i>Streptococcus mutans</i> (43.8%, <i>P</i> = 0.006), <i>Streptococcus sobrinus</i> (62.5%, <i>P</i> < 0.001), <i>Ligilactobacillus salivarius</i> (93.6%, <i>P</i> = 0.01) and <i>Candida albicans</i> (56.3%, <i>P</i> < 0.001) were significantly higher in the ECC group. The prevalence of ECC was higher in children with two targeted species present compared with children with one targeted species. Histidine metabolism and branched-chain amino acids degradation were activated in ECC group, while glyoxylate and dicarboxylate metabolism, purine and pyrimidine metabolism were inhibited. Histidine and glutathione metabolism was activated with enrichment of targeted microbial species, while linoleic acid metabolism and biotin metabolism was inhibited. The duration of each toothbrushing was a significant risk factor for ECC experience.</p><p><strong>Conclusion: </strong>The prevalence of <i>Scardovia wiggsiae, Streptococcus mutans, Streptococcus sobrinus</i> and <i>Candida albicans</i> is higher in ECC children compared to caries-free children. Oral habits and salivary metabolites also vary between ECC and caries-free children.</p>","PeriodicalId":19799,"journal":{"name":"PeerJ","volume":"13 ","pages":"e19399"},"PeriodicalIF":2.3000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063607/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"PeerJ","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.7717/peerj.19399","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Early childhood caries (ECC) has been proposed to be associated with various microorganisms and metabolites. This study aims to compare the prevalence of specific microbial species and salivary metabolomics profile in children with and without ECC, and to explore the correlation between salivary metabolites and targeted microbes.
Method: Five ml of unstimulated saliva was collected from 32 ECC and 22 caries-free children. Clinical indexed were recorded and questionnaires regarding oral health and dietary habits were obtained from the guardians. The presence of eight specific microbial species were examined using species-specific quantitative PCR (qPCR). Untargeted metabolomics was analyzed to identify key differential metabolites and pathways. Correlations among clinical, microbial, and metabolomic data were further explored.
Results: The prevalence of Scardovia wiggsiae (90.6%, P < 0.001), Streptococcus mutans (43.8%, P = 0.006), Streptococcus sobrinus (62.5%, P < 0.001), Ligilactobacillus salivarius (93.6%, P = 0.01) and Candida albicans (56.3%, P < 0.001) were significantly higher in the ECC group. The prevalence of ECC was higher in children with two targeted species present compared with children with one targeted species. Histidine metabolism and branched-chain amino acids degradation were activated in ECC group, while glyoxylate and dicarboxylate metabolism, purine and pyrimidine metabolism were inhibited. Histidine and glutathione metabolism was activated with enrichment of targeted microbial species, while linoleic acid metabolism and biotin metabolism was inhibited. The duration of each toothbrushing was a significant risk factor for ECC experience.
Conclusion: The prevalence of Scardovia wiggsiae, Streptococcus mutans, Streptococcus sobrinus and Candida albicans is higher in ECC children compared to caries-free children. Oral habits and salivary metabolites also vary between ECC and caries-free children.
期刊介绍：
PeerJ is an open access peer-reviewed scientific journal covering research in the biological and medical sciences. At PeerJ, authors take out a lifetime publication plan (for as little as $99) which allows them to publish articles in the journal for free, forever. PeerJ has 5 Nobel Prize Winners on the Board; they have won several industry and media awards; and they are widely recognized as being one of the most interesting recent developments in academic publishing.
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