Endophytic mycobiont provides growth benefits via a phenylpropanoid-auxin axis in host plants.

IF 7.5 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2025-05-05 DOI:10.1016/j.celrep.2025.115648

Cheng-Yen Chen, Naweed I Naqvi

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引用次数: 0

Abstract

Beneficial association with symbiotic fungi helps improve growth and fitness in most land plants and shows great potential as biofertilizers in precision agriculture. Here, we demonstrated that a root fungal endophyte, Tinctoporellus species isolate AR8, enabled yield improvement in Brassicaceae leafy green choy sum (Brassica rapa var. parachinensis). Mechanistically, AR8 colonized the root cortex/endosphere and channeled the metabolic flux to phenylpropanoids and requisite secondary metabolites to promote plant growth. AR8-assisted biosynthesis of auxin improved root growth and provided an intrinsic source for long-distance signaling that enhanced shoot biomass. Chemical complementation with exogenous p-coumaric acid restored auxin signaling and enhanced growth in AR8-inoculated pal1 mutant plants, thus implicating such a phenylpropanoid-auxin nexus as a pivotal regulator of symbiotic plant growth. Comparative metabolomics established hydroxycinnamic acid and p-coumaric acid as major plant-growth-promoting hubs that bridge the phenylpropanoid pathway and auxin signaling in the cross-kingdom AR8 symbiotic interaction model.

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内生真菌通过类苯丙素-生长素轴在寄主植物中提供生长益处。

与共生真菌的有益结合有助于改善大多数陆地植物的生长和适应性，并在精准农业中显示出巨大的生物肥料潜力。在这里，我们证明了根真菌内生菌，Tinctoporellus种分离物AR8，能够提高芸苔科绿叶白菜（Brassica rapa var. parachinensis）的产量。机理上，AR8定殖于根皮质/内球层，并将代谢通量导向苯丙素和必要的次生代谢物，以促进植物生长。ar8辅助生长素的生物合成促进了根的生长，并为长距离信号传递提供了内在来源，从而增加了茎部生物量。在ar8接种的pal1突变体植株中，外源对香豆酸的化学互补恢复了生长素信号并促进了生长，从而暗示这种苯丙素-生长素联系是共生植物生长的关键调节剂。比较代谢组学发现，羟基肉桂酸和对香豆酸是植物生长促进的主要枢纽，在跨界AR8共生相互作用模型中，它们连接了苯丙素途径和生长素信号通路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.