Assessment of Electrophoretic Mobility Determination in Nanoparticle Analysis: Two Parallel Techniques Converging in a Distinctive Parameter.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2025-04-29 DOI:10.1002/elps.202400132

Carlos Adelantado, Jan Jordens, Stefan Voorspoels, Milica Velimirovic, Kristof Tirez

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Abstract

A critical comparison of the main parameters playing a role in measurement of electrophoretic mobility of plastic nanoparticles (NPs) by CE and laser Doppler velocimetry (LDV) techniques in NP suspensions is herein presented, accompanied by a discussion about potential impact on different mobility values observed. Capillary material and dynamic or permanent coating of the inner capillary wall, capillary dimensions, EOF variability, BGE temperature, Joule heating, and presence of species potentially interacting with analyzed NPs are underlined as possible causes of the different performance of the above two techniques. It is of importance to get an insight into the reasons behind experimental conditions and operating features to opt for one technique or the other based on research interests. In the end, it is intended to present a knowledge expansion about two parallel paths that converge in a distinctive parameter of an enormous relevance in CE, effective electrophoretic mobility, not achievable by other techniques, and discuss practical considerations in experimental design.

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纳米颗粒分析中电泳迁移率测定的评估：两个平行的技术在一个独特的参数收敛。

本文介绍了用CE和激光多普勒测速（LDV）技术测量塑料纳米颗粒（NPs）在NP悬浮液中电泳迁移率的主要参数，并讨论了对观察到的不同迁移率值的潜在影响。毛细管材料和毛细管内壁的动态或永久涂层、毛细管尺寸、EOF变异性、BGE温度、焦耳加热以及可能与分析的NPs相互作用的物种的存在都被强调为上述两种技术不同性能的可能原因。重要的是要了解实验条件和操作特点背后的原因，根据研究兴趣选择一种技术或另一种技术。最后，它的目的是提出关于两个平行路径的知识扩展，这些路径在CE中具有巨大相关性的独特参数中收敛，有效的电泳迁移率，这是其他技术无法实现的，并讨论实验设计中的实际考虑因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.