Effects of Integrin-Linked Kinase Silencing Combined With Trichostatin A on Cancer Stem Cells.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-05-13 DOI:10.1111/odi.15377

Yulu Wu, Zhiyong Guo, Zhangao Li, Chenchen Cai, Jiyuan Liu, Xiufa Tang, Lin Que

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引用次数: 0

Abstract

Objectives: Oral squamous cell carcinoma (OSCC) is characterized by high invasiveness and metastasis, with cancer stem cells (CSCs) playing a central role in tumor progression. This study investigates the effects of integrin-linked kinase (ILK) silencing and trichostatin A (TSA) treatment on CSCs, assessing their potential to diminish CSC properties and inhibit OSCC progression.

Methods and materials: CSCs were enriched and isolated from primary OSCC samples and Tca8113 cell line and MOC1 cell line using side population (SP) analysis, with their characteristics and the therapeutic impact of treatments assessed through assays such as MTT, wound healing, cell invasion, cell cycle, and apoptosis.

Results: Higher SP cell content correlated significantly with poor pathological classification, metastasis, and recurrence. Treated CSCs showed reduced proliferation, migration, and invasion, along with increased apoptosis. In vivo experiments demonstrated that the combined treatment substantially reduced tumor growth.

Conclusion: The study confirms the efficacy of targeting CSCs with ILK silencing and TSA treatment in OSCC, suggesting a promising strategy for CSC-directed therapies that merit further investigation.

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整合素连接激酶沉默联合曲古霉素A对肿瘤干细胞的影响。

目的：口腔鳞状细胞癌（OSCC）具有高侵袭性和转移性的特点，肿瘤干细胞（CSCs）在肿瘤进展中起着核心作用。本研究探讨了整合素连接激酶（ILK）沉默和曲古斯汀A （TSA）治疗对CSC的影响，评估了它们降低CSC特性和抑制OSCC进展的潜力。方法和材料：采用侧群体（SP）分析方法，从原代OSCC样品和Tca8113细胞系、MOC1细胞系中富集分离CSCs，通过MTT、创面愈合、细胞侵袭、细胞周期、凋亡等试验评估CSCs的特性和治疗效果。结果：SP细胞含量高与病理分型差、转移及复发有显著相关性。处理后的CSCs增殖、迁移和侵袭减少，细胞凋亡增加。体内实验表明，联合治疗可显著降低肿瘤生长。结论：该研究证实了靶向CSCs的ILK沉默和TSA治疗OSCC的有效性，为csc定向治疗提供了一个有希望的策略，值得进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.