Efficacy of Nanoemulsified Benzyl Isothiocyanate and Carvacrol in Reducing Salmonella Contamination in Alfalfa Seeds and Sprouts.

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Sookyung Oh, Jitendra Patel
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Abstract

This study evaluated the efficacy of nanoemulsified benzyl isothiocyanate (NBIT) and carvacrol (NCR) to reduce Salmonella contamination on alfalfa seeds and sprouts. Given the susceptibility of sprouts to microbial contamination during germination, improved interventions were needed to enhance food safety without compromising product quality. NBIT and NCR were applied to Salmonella-inoculated seeds at high (6.3 log CFU/g) and low (2.4 log CFU/g) inoculum levels and their impact on Salmonella populations, seed germination, sprout quality (length and weight), and visual appearance was assessed. Results revealed that both NBIT and NCR significantly reduced Salmonella populations and most treatments did not adversely affect seed germination or sprout quality. Treatment with 2.0% NBIT achieved reductions of up to 2.4 log CFU/g on seeds and 6.4 log CFU/g on sprouts after 4 days at the low inoculum level. However, treatment with 2.0% NBIT resulted in an 18.8% reduction in germination that occurred at the high inoculum level and a 10.7% reduction at the low inoculum level, along with a slight decrease in sprout size. In contrast, treatments with 0.5% and 1.0% NBIT, as well as 2.0% NCR, reduced Salmonella on seeds by 1.5-2.0 log CFU/g, regardless of inoculum level, and achieved reductions of 5.0-6.4 log CFU/g on sprouts at the low inoculum level. In addition, NBIT (0.5% and 1.0%) and NCR (2.0%) preserved seed germination rates and sprout quality metrics, including length, weight, and visual appearance, comparable with untreated controls. These findings highlight the potential of NBIT and NCR as effective antimicrobial agents for improving the microbial safety of alfalfa sprouts without compromising quality.

纳米乳化异硫氰酸苄酯和香芹醇降低苜蓿种子和芽中沙门氏菌污染的效果。
本研究评价了纳米乳化异硫氰酸苄酯(NBIT)和香芹酚(NCR)对苜蓿种子和豆芽沙门氏菌污染的抑制效果。鉴于芽在萌发过程中对微生物污染的敏感性,需要改进干预措施,在不影响产品质量的情况下提高食品安全。将NBIT和NCR分别应用于高(6.3 log CFU/g)和低(2.4 log CFU/g)接种水平的沙门氏菌接种种子,并评估其对沙门氏菌种群、种子萌发、发芽质量(长度和重量)和视觉外观的影响。结果表明,NBIT和NCR处理均能显著降低沙门氏菌数量,且大多数处理对种子萌发和发芽质量没有不利影响。在低接种量下,2.0% NBIT处理4天后,种子减少了2.4 log CFU/g,芽减少了6.4 log CFU/g。然而,2.0% NBIT处理导致高接种量下发芽率下降18.8%,低接种量下发芽率下降10.7%,同时芽大小略有下降。相比之下,在0.5%和1.0% NBIT以及2.0% NCR处理下,无论接种量如何,种子上的沙门氏菌减少了1.5-2.0 log CFU/g,在低接种量下,芽上的沙门氏菌减少了5.0-6.4 log CFU/g。此外,NBIT(0.5%和1.0%)和NCR(2.0%)保存的种子发芽率和发芽质量指标,包括长度、重量和视觉外观,与未处理的对照组相当。这些发现突出了NBIT和NCR作为有效抗菌剂的潜力,可以在不影响质量的情况下提高苜蓿芽的微生物安全性。
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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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