Somaya Saad, Ahmed F Abouelnaga, Marwa Abass, Walaa Obydah, Nedaa A Kiwan, Omar Abd-Alhakem Ammar, Osama A Abulseoud, Abdelaziz M Hussein
{"title":"Effect of deep brain stimulation for lateral hypothalamic area on memory decline and hippocampal neurofilaments expression dysfunctions in aged rats.","authors":"Somaya Saad, Ahmed F Abouelnaga, Marwa Abass, Walaa Obydah, Nedaa A Kiwan, Omar Abd-Alhakem Ammar, Osama A Abulseoud, Abdelaziz M Hussein","doi":"10.1097/WNR.0000000000002162","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Studying the effect of deep brain stimulation (DBS) in the lateral hypothalamic area (LHA) in young and aging rats regarding memory changes, hippocampal neuronal dystrophy, and neurofilament expression.</p><p><strong>Methods: </strong>Thirty-six male Sprague-Dawley rats were divided into two main groups: adult young ( n = 18, 8 weeks old) and aged ( n = 18, 24 months old). Each main group was further subdivided into three equal subgroups ( n = 6) including control, sham, and DBS. DBS of LHA was conducted using high-frequency electric currents (130 Hz) for 1.5 h with 5-min breaks every 30 min for five consecutive days. Assessment of working memory was done using passive avoidance test (PAT). Then, the brain was dissected and hippocampal neuronal dystrophic damage was assessed as well as immunohistochemical examination of neurofilaments (NF68, NF200) expression.</p><p><strong>Results: </strong>Aging rats had progressive hippocampal neuronal degeneration and downregulation of heavy and light chain neurofilaments, that was associated with progressive decline in working memory. Nevertheless, activation of DBS in the LHA enhanced memory function as it increased latency to entry in PAT ( P < 0.001) compared to old normal and sham groups. Dystrophic damage score significantly decreased with DBS ( P < 0.001) in the hippocampal CA1, CA3, and dentate gyrus regions. Moreover, DBS upregulated hippocampal NF68, NF200 expression ( P < 0.001) in both young and old rats. We also found a significant positive correlation between working memory and NFs expression and a negative correlation between dystrophic damage score and NFs expression.</p><p><strong>Conclusions: </strong>DBS in the LHA may have a neuroprotective effect in aging rats as it enhanced the working memory and decreased hippocampal neuronal dystrophy. This protective effect may be caused by the upregulation of neurofilaments.</p>","PeriodicalId":19213,"journal":{"name":"Neuroreport","volume":" ","pages":"402-411"},"PeriodicalIF":1.6000,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuroreport","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1097/WNR.0000000000002162","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/11 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: Studying the effect of deep brain stimulation (DBS) in the lateral hypothalamic area (LHA) in young and aging rats regarding memory changes, hippocampal neuronal dystrophy, and neurofilament expression.
Methods: Thirty-six male Sprague-Dawley rats were divided into two main groups: adult young ( n = 18, 8 weeks old) and aged ( n = 18, 24 months old). Each main group was further subdivided into three equal subgroups ( n = 6) including control, sham, and DBS. DBS of LHA was conducted using high-frequency electric currents (130 Hz) for 1.5 h with 5-min breaks every 30 min for five consecutive days. Assessment of working memory was done using passive avoidance test (PAT). Then, the brain was dissected and hippocampal neuronal dystrophic damage was assessed as well as immunohistochemical examination of neurofilaments (NF68, NF200) expression.
Results: Aging rats had progressive hippocampal neuronal degeneration and downregulation of heavy and light chain neurofilaments, that was associated with progressive decline in working memory. Nevertheless, activation of DBS in the LHA enhanced memory function as it increased latency to entry in PAT ( P < 0.001) compared to old normal and sham groups. Dystrophic damage score significantly decreased with DBS ( P < 0.001) in the hippocampal CA1, CA3, and dentate gyrus regions. Moreover, DBS upregulated hippocampal NF68, NF200 expression ( P < 0.001) in both young and old rats. We also found a significant positive correlation between working memory and NFs expression and a negative correlation between dystrophic damage score and NFs expression.
Conclusions: DBS in the LHA may have a neuroprotective effect in aging rats as it enhanced the working memory and decreased hippocampal neuronal dystrophy. This protective effect may be caused by the upregulation of neurofilaments.
期刊介绍：
NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool.
The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works.
We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
