In Vitro Anti-Hepatitis B Virus Activity of Hydroxytyrosol from Lindernia ruellioides.

IF 4.2 2区化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules Pub Date : 2025-05-06 DOI:10.3390/molecules30092063

Tong-Shi-Yao Zhao, Kang-Zhi Li, He-Ling Su, Bin Liang, Cheng-Qin Liang, Jin-Tao Gao, Xian-Li Zhou

{"title":"In Vitro Anti-Hepatitis B Virus Activity of Hydroxytyrosol from Lindernia ruellioides.","authors":"Tong-Shi-Yao Zhao, Kang-Zhi Li, He-Ling Su, Bin Liang, Cheng-Qin Liang, Jin-Tao Gao, Xian-Li Zhou","doi":"10.3390/molecules30092063","DOIUrl":null,"url":null,"abstract":"Hepatitis B is a serious infectious disease that threatens the health of all mankind. In this study, we isolated and extracted hydroxytyrosol from Lindernia ruellioides with anti-hepatitis B virus (HBV) activity. The structure of hydroxytyrosol was identified by the nuclear magnetic resonance technique. HepG2.2.15 cell models were used to detect the anti-HBV activity and liver protection of hydroxytyrosol in vitro. Hydroxytyrosol can inhibit hepatitis B surface antigen (HBsAg) and hepatitis B e-antigen (HBeAg). The IC50 values of HBsAg and HBeAg were 4.02 mg/L and 5.19 mg/L, respectively. At the highest concentration of hydroxytyrosol, the inhibition rates of supernatant and intracellular HBV DNA were 75.99% and 66.33%, respectively. Hydroxytyrosol was less toxic to normal human hepatocytes. Molecular docking showed that hydroxytyrosol was bound to three amino acid residues of HBV polymerase with a binding energy of -7.0 kcal/mol. This study provided data for the development and utilization of Lindernia ruellioides and the research and development of anti-hepatitis B virus drugs.","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 9","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12073499/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecules","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.3390/molecules30092063","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Hepatitis B is a serious infectious disease that threatens the health of all mankind. In this study, we isolated and extracted hydroxytyrosol from Lindernia ruellioides with anti-hepatitis B virus (HBV) activity. The structure of hydroxytyrosol was identified by the nuclear magnetic resonance technique. HepG2.2.15 cell models were used to detect the anti-HBV activity and liver protection of hydroxytyrosol in vitro. Hydroxytyrosol can inhibit hepatitis B surface antigen (HBsAg) and hepatitis B e-antigen (HBeAg). The IC₅₀ values of HBsAg and HBeAg were 4.02 mg/L and 5.19 mg/L, respectively. At the highest concentration of hydroxytyrosol, the inhibition rates of supernatant and intracellular HBV DNA were 75.99% and 66.33%, respectively. Hydroxytyrosol was less toxic to normal human hepatocytes. Molecular docking showed that hydroxytyrosol was bound to three amino acid residues of HBV polymerase with a binding energy of -7.0 kcal/mol. This study provided data for the development and utilization of Lindernia ruellioides and the research and development of anti-hepatitis B virus drugs.

查看原文本刊更多论文

枸杞子羟基酪醇体外抗乙型肝炎病毒活性的研究。

乙型肝炎是威胁全人类健康的严重传染病。在本研究中，我们分离并提取了具有抗乙型肝炎病毒（HBV）活性的红叶连翘（Lindernia ruellioides）羟酪醇。利用核磁共振技术鉴定了羟基酪醇的结构。采用HepG2.2.15细胞模型检测羟基酪醇体外抗hbv活性及肝脏保护作用。羟基酪醇具有抑制乙型肝炎表面抗原（HBsAg）和乙型肝炎e抗原（HBeAg）的作用。HBsAg和HBeAg的IC50值分别为4.02 mg/L和5.19 mg/L。在羟基酪醇浓度最高时，上清液和细胞内HBV DNA的抑制率分别为75.99%和66.33%。羟基酪醇对正常人肝细胞毒性较小。分子对接表明，羟基酪醇与HBV聚合酶的3个氨基酸残基结合，结合能为-7.0 kcal/mol。本研究为瑞莲的开发利用和抗乙型肝炎病毒药物的研究开发提供了依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecules 化学-有机化学

CiteScore

7.40

自引率

8.70%

发文量

7524

审稿时长

1.4 months

期刊介绍： Molecules (ISSN 1420-3049, CODEN: MOLEFW) is an open access journal of synthetic organic chemistry and natural product chemistry. All articles are peer-reviewed and published continously upon acceptance. Molecules is published by MDPI, Basel, Switzerland. Our aim is to encourage chemists to publish as much as possible their experimental detail, particularly synthetic procedures and characterization information. There is no restriction on the length of the experimental section. In addition, availability of compound samples is published and considered as important information. Authors are encouraged to register or deposit their chemical samples through the non-profit international organization Molecular Diversity Preservation International (MDPI). Molecules has been launched in 1996 to preserve and exploit molecular diversity of both, chemical information and chemical substances.