In Vitro Assessment of Anthelmintic Activities of AgO Nanoparticle against Liver Fluke Dicrocoelium dendriticum.

IF 0.9 4区医学 Q4 PARASITOLOGY

Iranian Journal of Parasitology Pub Date : 2025-01-01 DOI:10.18502/ijpa.v20i1.18103

Mohsen Arbabi, Atefeh Haddad, Seyed Mostafa Hosseipour Mashkani, Hossein Hooshyar

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引用次数: 0

Abstract

Background: Dicrocoeliasis is an important helminthic zoonosis reported from many parts of the world. Due to low-performance medications, drug delivery is a great challenge in improving the treatment of this liver fluke infection. We aimed to determine the anthelmintic properties of Nanosilver oxide (AgO) against Dicrocoelium dendriticum infection.

Methods: The impacts of various concentrations of AgO nanoparticles (50-200 μg/ml) for 12-24 hours were compared with closantel, a chemical drug. The anthelmintic efficacy was evaluated using the scanning electron microscopy (SEM) technique. The synthesized nanoparticles were analyzed for structural assessment using XRD, UV-VIS spectroscopy, and SEM. The XRD pattern shows the formation of AgO nanoparticles.

Results: The UV-VIS spectra showed the broad peak, corresponding to Ag nanoparticles. SEM images of treated parasites by AgO (200 μg/ml) showed severe damage, which includes complete loss of sensory papillae and destruction of prominent network structures and tegument vesicles. The mortality rate increases with the increase in the concentration and exposure time of the parasite to nanoparticles. Besides the MTT assay, the toxicity of AgO, at concentrations of 800 μg/ml was 8.7%.

Conclusion: AgO NPs have potent anthelmintic effects on liver fluke D. dendriticum. This is the first research that assessed the effect of AgO NP on liver fluke D. dendriticum. Hence, the present study provides a basis for future research on the control of this common trematode.

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纳米AgO颗粒对树突肝吸虫驱虫活性的体外研究。

背景：双角绦虫病是一种重要的寄生虫病，世界上许多地方都有报道。由于药物的低效能，给药是改善这种肝吸虫感染治疗的一大挑战。我们的目的是确定纳米氧化银（AgO）对树突双星感染的驱虫特性。方法：比较不同浓度AgO纳米颗粒（50 ~ 200 μg/ml）对化学药物closantel作用12 ~ 24 h的影响。采用扫描电镜（SEM）技术评价其驱虫效果。采用XRD、UV-VIS光谱和SEM对合成的纳米颗粒进行了结构分析。XRD图显示了AgO纳米颗粒的形成。结果：紫外可见光谱为宽峰，与银纳米粒子相对应。经200 μg/ml AgO处理的寄生虫，其SEM图像显示出严重的损伤，包括感觉乳头完全丧失，突出的网状结构和被皮囊泡被破坏。随着纳米粒子的浓度和暴露时间的增加，寄生虫的死亡率也随之增加。除MTT法外，AgO浓度为800 μg/ml时毒性为8.7%。结论：AgO NPs对树突肝吸虫有较强的驱虫作用。这是第一个评估AgO NP对树突肝吸虫影响的研究。因此，本研究为今后对该常见吸虫的防治研究提供了基础。

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来源期刊

Iranian Journal of Parasitology PARASITOLOGY-

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.