Assessment of the Validity of Carbon Ion Irradiation for C6 Gliomas in Rats.

IF 2.4 4区医学 Q3 PHARMACOLOGY & PHARMACY

Dose-Response Pub Date : 2025-04-15 eCollection Date: 2025-04-01 DOI:10.1177/15593258251327505

Yufeng Li, Lei Ta, QingFeng Wu, Hongyu Zhang, Yuan Xu, Lu Gan, Jianli Liu

{"title":"Assessment of the Validity of Carbon Ion Irradiation for C6 Gliomas in Rats.","authors":"Yufeng Li, Lei Ta, QingFeng Wu, Hongyu Zhang, Yuan Xu, Lu Gan, Jianli Liu","doi":"10.1177/15593258251327505","DOIUrl":null,"url":null,"abstract":"Purpose: Application of energy-spectrum computed tomography (CT) to assess specific efficacy of and response to carbon ion radiotherapy (CIRT) of C6 gliomas in rats.Methods: After establishing C6 glioma rat models, 3 tumor-bearing rats were randomly selected as controls. The remaining were divided into 0 Gy, 1 Gy, and 2 Gy groups for CIRT. Energy-spectrum CT scans were performed, and brain tissues were collected for histopathology and western blot Test. Survival rates in each group were compared.Results: The results demonstrated that tumors in the 1 Gy and 2 Gy groups decreased at different rates up to 14 days post-CIRT (P < 0.05). Furthermore, compared to pre-CIRT measurements, the energy-spectrum parameters gradually increased in the 0 Gy group, while they decreased in the 2 Gy group. Post-CIRT, the Ki-67 proliferation index and the expression levels of vascu-larassociated proteins in tumor tissues were significantly reduced in the 1 and 2 Gy groups. Additionally, the survival times of tumor-bearing rats were prolonged after CIRT.Conclusions: CIRT effectively restricts tumor cell growth and proliferation, leading to improved survival rates in rats with C6 gliomas. The use of energy-spectrum CT with immunohistochemistry for quantitative detection can actively support the effectiveness of carbon ion radiotherapy in inhibiting tumor proliferation.","PeriodicalId":11285,"journal":{"name":"Dose-Response","volume":"23 2","pages":"15593258251327505"},"PeriodicalIF":2.4000,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12033543/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dose-Response","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/15593258251327505","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: Application of energy-spectrum computed tomography (CT) to assess specific efficacy of and response to carbon ion radiotherapy (CIRT) of C6 gliomas in rats.

Methods: After establishing C6 glioma rat models, 3 tumor-bearing rats were randomly selected as controls. The remaining were divided into 0 Gy, 1 Gy, and 2 Gy groups for CIRT. Energy-spectrum CT scans were performed, and brain tissues were collected for histopathology and western blot Test. Survival rates in each group were compared.

Results: The results demonstrated that tumors in the 1 Gy and 2 Gy groups decreased at different rates up to 14 days post-CIRT (P < 0.05). Furthermore, compared to pre-CIRT measurements, the energy-spectrum parameters gradually increased in the 0 Gy group, while they decreased in the 2 Gy group. Post-CIRT, the Ki-67 proliferation index and the expression levels of vascu-larassociated proteins in tumor tissues were significantly reduced in the 1 and 2 Gy groups. Additionally, the survival times of tumor-bearing rats were prolonged after CIRT.

Conclusions: CIRT effectively restricts tumor cell growth and proliferation, leading to improved survival rates in rats with C6 gliomas. The use of energy-spectrum CT with immunohistochemistry for quantitative detection can actively support the effectiveness of carbon ion radiotherapy in inhibiting tumor proliferation.

Abstract Image

查看原文本刊更多论文

碳离子辐照治疗大鼠C6胶质瘤的有效性评价。

目的：应用能谱计算机断层扫描（CT）评价碳离子放疗（CIRT）治疗大鼠C6胶质瘤的特异性疗效和反应。方法：建立C6胶质瘤大鼠模型后，随机选取3只荷瘤大鼠作为对照。其余分为0 Gy、1 Gy和2 Gy组进行CIRT。行能谱CT扫描，采集脑组织进行组织病理学和western blot检测。比较各组患者的生存率。结果：1 Gy组和2 Gy组的肿瘤在cirt后14天内均有不同程度的缩小（P < 0.05）。此外，与预cirt测量相比，能量谱参数在0 Gy组逐渐增加，而在2 Gy组则逐渐减少。cirt后，1、2 Gy组肿瘤组织Ki-67增殖指数及血管相关蛋白表达水平均显著降低。此外，CIRT可延长荷瘤大鼠的生存时间。结论：CIRT有效地抑制了肿瘤细胞的生长和增殖，提高了C6胶质瘤大鼠的存活率。利用能谱CT结合免疫组织化学进行定量检测，可以积极支持碳离子放疗抑制肿瘤增殖的有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Dose-Response PHARMACOLOGY & PHARMACY-RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

CiteScore

4.90

自引率

4.00%

发文量

140

审稿时长

>12 weeks

期刊介绍： Dose-Response is an open access peer-reviewed online journal publishing original findings and commentaries on the occurrence of dose-response relationships across a broad range of disciplines. Particular interest focuses on experimental evidence providing mechanistic understanding of nonlinear dose-response relationships.