Identification and antimicrobial susceptibility testing of Streptococcus agalactiae associated urinary tract infections using VITEK 2 system.

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2025-04-01 DOI:10.18502/ijm.v17i2.18383

Parviz Mohajeri, Hossein Faridafshar, Sara Kooti

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引用次数: 0

Abstract

Background and objectives: As a Gram-positive bacterium, Streptococcus agalactiae or Group B Streptococcus (GBS) is normally found as a transient flora of the gastrointestinal and genitourinary tracts of women. The high prevalence of GBS in the urethra warrants investigation of UTIs and antibiotic resistance frequency associated with GBS. Given the paucity of research on antibiotic resistance of GBS in Iran, the present study investigated the UTIs associated with GBS and the antibiotic susceptibility patterns associated with GBS.

Materials and methods: This study included 65 GBS strains collected from urine samples obtained from the Bouali Laboratory Complex, one of the largest laboratories in western Iran. VITEK 2 GP ID cards were used to identify all GBS isolates. VITEK 2 susceptibility testing for Gram-positive bacteria was performed according to the manufacturer's instructions using the AST-ST card. MIC method was performed after the detection of GBS strains.

Results: We found that 53 (81.5%) of the GBS isolates showed resistance to tetracycline; 47 (72.3%), 40 (61.5%), and 30 (46.15%) of these had a resistance to erythromycin, clindamycin and ampicillin respectively.

Conclusion: In the present study, the VITEK 2 system was validated as a user-friendly system that can serve as a rapid and accurate tool for identification and antimicrobial susceptibility testing of GBS.

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VITEK 2系统检测无乳链球菌相关性尿路感染及药敏试验。

背景和目的：作为一种革兰氏阳性细菌，无乳链球菌或B族链球菌（GBS）通常作为女性胃肠道和泌尿生殖道的短暂菌群被发现。尿道中GBS的高患病率值得调查尿路感染和与GBS相关的抗生素耐药频率。鉴于伊朗对GBS抗生素耐药性的研究不足，本研究调查了与GBS相关的uti和与GBS相关的抗生素敏感性模式。材料和方法：本研究包括从伊朗西部最大的实验室之一Bouali综合实验室的尿液样本中收集的65株GBS菌株。使用VITEK 2 GP ID卡对所有GBS分离株进行鉴定。根据制造商的说明使用AST-ST卡进行革兰氏阳性细菌的VITEK 2药敏试验。检测GBS菌株后采用MIC法。结果：53株GBS（81.5%）对四环素耐药；其中对红霉素耐药47例（72.3%），克林霉素耐药40例（61.5%），氨苄青霉素耐药30例（46.15%）。结论：VITEK 2系统是一种用户友好的系统，可作为快速准确的GBS鉴定和药敏试验工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.