Study on the Inhibitory Effects of Three Endophytic Bacillus Strains on Aspergillus flavus in Maize.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2025-04-11 DOI:10.3390/metabo15040268

Siyu Ma, Min Li, Siqi Zhang, Yin Yang, Fengsha Zhu, Xingyu Li, Shahzad Munir, Pengfei He, Pengbo He, Yixin Wu, Yueqiu He, Ping Tang

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引用次数: 0

Abstract

Background: Maize is easily contaminated by Aspergillus flavus, and the aflatoxin produced by A. flavus has been classified as a Group 1 carcinogen, for which there are currently no effective control measures. Biological control is regarded as an environmentally friendly and safe approach. Strains ZH179, ZH409, and ZH99 are three bacteria isolated from our laboratory that exhibit antagonistic effects against A. flavus. We conducted experiments to investigate their biocontrol efficacy.

Results: The experimental results demonstrated that these three strains effectively inhibited A. flavus on plates and stored maize seeds. Identification revealed that ZH179 is Bacillus velezensis, while ZH409 and ZH99 are B. amyloliquefaciens. We also identified lipopeptide synthetase-related genes, including srfAA, srfAD, fenA, fenB, ituA, ituB, ituD, bmyA, bmyB, and bmyC, in these three strains. Furthermore, LC-MS analysis confirmed that these strains could produce lipopeptide compounds such as surfactin, fengycin, iturin, and bacillomycin. Using the Oxford cup method, we found that the lipopeptide compounds produced by these strains can inhibit the growth of A. flavus.

Conclusion: These findings suggest that strains ZH179, ZH409, and ZH99 have good control effects on A. flavus during the storage of maize, primarily due to the lipopeptide compounds. This study provides a theoretical basis for using these three strains in the biological control of A. flavus.

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3株内生芽孢杆菌对玉米黄曲霉抑制作用的研究。

背景：玉米容易被黄曲霉污染，黄曲霉产生的黄曲霉毒素已被列为1类致癌物，目前尚无有效的控制措施。生物防治被认为是一种环境友好和安全的方法。菌株ZH179、ZH409和ZH99是本实验室分离的三种对黄芽孢杆菌具有拮抗作用的细菌。我们对其生物防治效果进行了实验研究。结果：实验结果表明，这3株菌株对平板和贮藏玉米种子的黄曲霉有较好的抑制作用。鉴定结果显示，ZH179为velezensis芽孢杆菌，ZH409和ZH99为解淀粉芽孢杆菌。我们还在这三种菌株中鉴定了脂肽合成酶相关基因，包括srfAA、srfAD、fenA、fenB、ituA、ituB、ituD、bmyA、bmyB和bmyC。此外，LC-MS分析证实这些菌株可以产生脂肽类化合物，如表面素、风霉素、iturin和杆菌霉素。使用牛津杯法，我们发现这些菌株产生的脂肽化合物可以抑制黄曲霉的生长。结论：菌株ZH179、ZH409和ZH99在玉米贮藏过程中对黄曲霉有较好的防治作用，主要是由于其脂肽类化合物的作用。本研究为利用这3株菌株对黄曲霉进行生物防治提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.