Rotavirus prevalence and genotypes in the Central African Republic, 2011-2021.

IF 3.4 3区医学 Q2 INFECTIOUS DISEASES

BMC Infectious Diseases Pub Date : 2025-05-08 DOI:10.1186/s12879-025-11057-4

Joël W Dote, Virginie Banga Mingo, Jean Fandema, Jean C Gody, Jason M Mwenda, Mathew D Esona, Michael D Bowen, Narcisse Patrice J Komas, Ionela Gouandjika-Vasilache, Diane Waku-Kouomou

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引用次数: 0

Abstract

Background: Rotavirus gastroenteritis is one of major causes of death in infants, particularly in sub-Saharan Africa. In the Central African Republic (CAR), sentinel surveillance of rotavirus gastroenteritis was established in 2011. In this study, we assessed the burden of rotavirus gastroenteritis and identified rotavirus strains circulating in CAR during 2011-2021.

Methods: Stool samples were collected from < 5-year-old children with diarrhoea according to WHO criteria, at the sentinel site in Bangui, CAR. Samples were screened for group A rotavirus antigen by EIA. RNA was extracted from all EIA-positive samples which were subjected to genotyping using a semi nested RT-PCR assay.

Results: From 2011 to 2021, 1855 stool samples were collected and 854 (46.0%) were positive for rotavirus by EIA. Genotypes were obtained from 77.3% (660/854) EIA positive samples. Of these 660 samples, genotypes funds were: G1 (35.4%) and G2 (26.6%) for VP7, and P[6] (42.7%) and P[8] (35.6%) for the VP4 gene. The most frequent genotype combinations were G1P[8], 19.3% and G1P[6], 15.0%.

Conclusion: This study reports the prevalence of rotavirus genotypes that circulated for ten years, providing a pre-vaccine baseline data genotype estimate for rotavirus gastroenteritis sentinel surveillance in the Central African Republic.

Clinical trial number: Not applicable.

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2011-2021年中非共和国轮状病毒流行率和基因型

背景：轮状病毒胃肠炎是婴儿死亡的主要原因之一，特别是在撒哈拉以南非洲。中非共和国于2011年建立了轮状病毒肠胃炎哨点监测。在这项研究中，我们评估了轮状病毒胃肠炎的负担，并确定了2011-2021年在中非流行的轮状病毒株。结果：2011 - 2021年共收集粪便标本1855份，经环检轮状病毒阳性854份（46.0%）。77.3% (660/854) EIA阳性标本获得基因型。在这660个样本中，VP7基因型基金分别为G1（35.4%）和G2 (26.6%)， VP4基因型基金为P[6]（42.7%）和P[8]（35.6%）。最常见的基因型组合是G1P[8]（19.3%）和G1P bb0（15.0%）。结论：本研究报告了流传十年的轮状病毒基因型的流行情况，为中非共和国轮状病毒胃肠炎哨点监测提供了疫苗前基线数据基因型估计。临床试验号：不适用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Infectious Diseases 医学-传染病学

CiteScore

6.50

自引率

0.00%

发文量

860

审稿时长

3.3 months

期刊介绍： BMC Infectious Diseases is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of infectious and sexually transmitted diseases in humans, as well as related molecular genetics, pathophysiology, and epidemiology.