Efficacy and Feasibility of Tissue-Clearing Technique and Three-Dimensional Imaging in the Human Gastrointestinal Tissues Using Illuminate Cleared Organs to Identify Target Molecules.
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引用次数: 0
Abstract
Introduction: Tissue-clearing technology has shown potential for comprehensive structural and functional analysis through three-dimensional (3D) imaging of biological tissue. However, its effectiveness in human specimens remains insufficiently explored. In this study, we validated the illuminate cleared organs to identify target molecules (LUCID) protocol for human gastrointestinal specimens and demonstrated its utility in enhancing tissue transparency and 3D imaging.
Methods: The gastrointestinal mucosa specimens resected via endoscopic submucosal dissection including the esophagus, stomach, duodenum, and colon were fluorescently stained and optically cleared using LUCID. Cleared specimens were imaged in 3D form by confocal laser scanning microscope, and the observable depth at any five points was measured and compared to non-cleared specimens, respectively. After clearing and imaging, the specimens were restored to the formalin-fixed paraffin-embedded form again, and conventional two-dimensional pathological evaluation using hematoxylin-eosin, Ki67, p53, and E-cadherin staining was performed to compare them with their preclearing state.
Results: The observable depth was significantly extended after clearing for specimens from each organ (esophagus 228.3 ± 14.9 µm vs. 1,036.7 ± 62.9 µm, p < 0.05; stomach 115.2 ± 5.5 µm vs. 428.7 ± 15.9 µm, p < 0.05; duodenum 256.2 ± 9.5 µm vs. 787.0 ± 18.6 µm, p < 0.05, colon 113.9 ± 5.4 µm vs. 436.6 ± 18.5 µm, p < 0.05). The pathological evaluation after clearing revealed a preserved fine structure and staining and showed no apparent deformation, degeneration, or tissue damage compared with before clearing.
Conclusions: The effectiveness of tissue clearing using LUCID on human gastrointestinal specimens was demonstrated, and the LUCID protocol had minimal impact on specimen morphology and staining. LUCID is expected to be a method that enables comprehensive structural analysis of human gastrointestinal mucosa and lesions that may avoid missing microscopic findings that may occur in split-face pathological assessment.
期刊介绍:
Each issue of this journal is dedicated to a special topic of current interest, covering both clinical and basic science topics in gastrointestinal function and disorders. The contents of each issue are comprehensive and reflect the state of the art, featuring editorials, reviews, mini reviews and original papers. These individual contributions encompass a variety of disciplines including all fields of gastroenterology. ''Digestive Diseases'' bridges the communication gap between advances made in the academic setting and their application in patient care. The journal is a valuable service for clinicians, specialists and physicians-in-training.