Synthesis of HBC fluorophores with an electrophilic handle for covalent attachment to Pepper RNA.

IF 2.2 4区化学 Q2 CHEMISTRY, ORGANIC

Beilstein Journal of Organic Chemistry Pub Date : 2025-04-04 eCollection Date: 2025-01-01 DOI:10.3762/bjoc.21.56

Raphael Bereiter, Ronald Micura

引用次数: 0

Abstract

The fluorescent light-up aptamer (FLAP) Pepper can utilize fluorophores that are equipped with an electrophilic handle for the covalent attachment of the surrogate to the RNA. The resulting irreversibly tethered dye-RNA complexes have opened up new avenues for RNA imaging in live cells. Here, we report the syntheses of such modified HBC530 ((4-((2-hydroxyethyl)(methyl)amino)benzylidene)cyanophenylacetonitrile) fluorophores for easy access, which will contribute to the rapid dissemination of the RNA imaging approaches associated therewith.

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与辣椒RNA共价连接的亲电柄HBC荧光团的合成。

荧光发光适体（FLAP） Pepper可以利用带有亲电柄的荧光团将替代物与RNA共价连接。由此产生的不可逆拴系染料-RNA复合物为活细胞中的RNA成像开辟了新的途径。在这里，我们报道了这种易于获取的修饰HBC530（（4-（（2-羟乙基）（甲基）氨基）苄基）氰苯乙腈）荧光团的合成，这将有助于与之相关的RNA成像方法的快速传播。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Beilstein Journal of Organic Chemistry 化学-有机化学

CiteScore

4.90

自引率

3.70%

发文量

167

审稿时长

1.4 months

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