A Rare Case of Anti-Kpa Antibody Identification in a Chinese Patient.

IF 0.7 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Clinical laboratory Pub Date : 2025-04-01 DOI:10.7754/Clin.Lab.2025.250212

Zengzhen Wei, Xiaohong Wang, Fu Cheng, Li Wang, Jinzhe Tan, Bin Tan

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引用次数: 0

Abstract

Background: Here we present a rare case of anti-Kpa alloantibody identified in a Chinese individual, along with a literature review.

Methods: An antibody identification test was conducted to clarify the antibody specificity. To further characterize this antibody, we assessed the reactivity of the patient's serum with relative antigen-positive red blood cells at different test phases. We also investigated the sensitivity of the antigen to DTT and papain. We separated the pa-tient's own RBCs from the transfused ones using capillary centrifugation and performed direct antiglobulin test and Kell blood group antigen detection.

Results: The patient's Kell phenotype was Kp(a-b+), and an IgG-specific anti-Kpa antibody was identified, resulting from the transfusion of Kp(a+b+) allogeneic red blood cells (RBCs). The Kpa antigen on RBCs was susceptible to 0.2M DTT but not to papain.

Conclusions: This case highlights the presence of an anti-Kpa antibody in the Chinese population, suggesting that a reassessment of the frequency of this antigen in this demographic is warranted.

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一例罕见的中国患者抗kpa抗体鉴定。

背景：在这里，我们报告了一例罕见的中国个体发现的抗kpa同种异体抗体，并进行了文献综述。方法：采用抗体鉴定试验，明确抗体特异性。为了进一步表征该抗体，我们评估了患者血清在不同测试阶段与相对抗原阳性红细胞的反应性。我们还研究了抗原对DTT和木瓜蛋白酶的敏感性。采用毛细管离心分离法将患者自身红细胞与输注红细胞分离，进行直接抗球蛋白试验和Kell血型抗原检测。结果：患者的Kell表型为Kp(a-b+)，并鉴定出igg特异性抗kpa抗体，这是由输注Kp（a+b+）异体红细胞（rbc）引起的。红细胞上Kpa抗原对0.2M DTT敏感，对木瓜蛋白酶不敏感。结论：该病例强调了中国人群中抗kpa抗体的存在，表明有必要重新评估该抗原在该人群中的频率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical laboratory 医学-医学实验技术

CiteScore

1.50

自引率

0.00%

发文量

494

审稿时长

3 months

期刊介绍： Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.