The application of metagenomic next generation sequencing in diagnosing tuberculous otitis media: a case report and review of the literature.

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL

Journal of Medical Case Reports Pub Date : 2025-05-05 DOI:10.1186/s13256-025-05257-4

HongYan Liu, YiFan Zhu, YangYiYi Huang, Hua Jiang

{"title":"The application of metagenomic next generation sequencing in diagnosing tuberculous otitis media: a case report and review of the literature.","authors":"HongYan Liu, YiFan Zhu, YangYiYi Huang, Hua Jiang","doi":"10.1186/s13256-025-05257-4","DOIUrl":null,"url":null,"abstract":"Background: Tuberculous otitis media is a chronic Mycobacterium tuberculosis infection of the middle ear tissues. Diseases with varied and insidious clinical features can make diagnosis difficult and delay treatment.Case presentation: Here, we document a case of tuberculous otitis media in a 46-year-old ethnic Han woman that manifested as nonspecific chronic otitis media. A mastoidectomy and tympanoplasty were performed for the initial diagnosis of cholesteatoma. The histopathology of the tissue specimen revealed granuloma formation with necrosis. Staining for acid-fast bacilli and the polymerase chain reaction method for Mycobacterium tuberculosis yielded negative results. However, the chest computed tomography scan demonstrated a pulmonary miliary nodule. Next, metagenomic next-generation sequencing was applied and the Mycobacterium tuberculosis was identified. The patient recovered after receiving antituberculous treatment.Conclusion: This report highlights the application of novel diagnostic tools such as metagenomic next-generation sequencing as a supplementary method for the diagnosis of tuberculous otitis media in highly suspected patients.","PeriodicalId":16236,"journal":{"name":"Journal of Medical Case Reports","volume":"19 1","pages":"207"},"PeriodicalIF":0.9000,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12051264/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Medical Case Reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s13256-025-05257-4","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Tuberculous otitis media is a chronic Mycobacterium tuberculosis infection of the middle ear tissues. Diseases with varied and insidious clinical features can make diagnosis difficult and delay treatment.

Case presentation: Here, we document a case of tuberculous otitis media in a 46-year-old ethnic Han woman that manifested as nonspecific chronic otitis media. A mastoidectomy and tympanoplasty were performed for the initial diagnosis of cholesteatoma. The histopathology of the tissue specimen revealed granuloma formation with necrosis. Staining for acid-fast bacilli and the polymerase chain reaction method for Mycobacterium tuberculosis yielded negative results. However, the chest computed tomography scan demonstrated a pulmonary miliary nodule. Next, metagenomic next-generation sequencing was applied and the Mycobacterium tuberculosis was identified. The patient recovered after receiving antituberculous treatment.

Conclusion: This report highlights the application of novel diagnostic tools such as metagenomic next-generation sequencing as a supplementary method for the diagnosis of tuberculous otitis media in highly suspected patients.

查看原文本刊更多论文

新一代宏基因组测序在诊断结核性中耳炎中的应用：1例报告及文献复习。

背景：结核性中耳炎是一种慢性结核分枝杆菌感染的中耳组织。疾病的各种和潜伏的临床特征可以使诊断困难和延误治疗。病例介绍：在这里，我们记录了一例46岁汉族女性的结核性中耳炎，表现为非特异性慢性中耳炎。乳突切除及鼓室成形术作为胆脂瘤的初步诊断。组织病理检查显示肉芽肿形成伴坏死。抗酸杆菌染色和结核分枝杆菌聚合酶链反应法均为阴性。然而，胸部计算机断层扫描显示肺军事结节。接下来，应用新一代宏基因组测序技术鉴定结核分枝杆菌。病人接受抗结核治疗后痊愈。结论：本报告强调了新一代宏基因组测序等新型诊断工具的应用，作为高度怀疑患者诊断结核性中耳炎的补充方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Medical Case Reports Medicine-Medicine (all)

CiteScore

1.50

自引率

0.00%

发文量

436

期刊介绍： JMCR is an open access, peer-reviewed online journal that will consider any original case report that expands the field of general medical knowledge. Reports should show one of the following: 1. Unreported or unusual side effects or adverse interactions involving medications 2. Unexpected or unusual presentations of a disease 3. New associations or variations in disease processes 4. Presentations, diagnoses and/or management of new and emerging diseases 5. An unexpected association between diseases or symptoms 6. An unexpected event in the course of observing or treating a patient 7. Findings that shed new light on the possible pathogenesis of a disease or an adverse effect