In Vitro Pharmacological Activity of Endophyte Aspergillus austwickii isolated from the Leaves of Premna serratifolia.

Q3 Veterinary
Archives of Razi Institute Pub Date : 2024-10-31 eCollection Date: 2024-10-01 DOI:10.32592/ARI.2024.79.5.1023
L V S, A K Shettar, J H Hoskeri, V A B
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引用次数: 0

Abstract

Diabetes mellitus is a widely occurring non-communicable disease that is rapidly spreading worldwide. It results from the dysregulation of glucose in the bloodstream or the improper functioning of the enzymes α-amylase and α-glucosidase. Endophytes are microorganisms that reside symbiotically within the living tissues of plants. Endophytic fungi possess the ability to synthesize various important bioactive metabolites. The current study aims to explore the less-reported endophytic fungus Aspergillus austwickii, isolated from the ethnobotanical medicinal plant Premna serratifolia L. The methanolic extract of the endophytic fungus was subjected to in vitro antioxidant assays, anti-inflammatory assays, and antidiabetic assays, in addition to analyzing the total phenolic and total flavonoid content, along with the phytochemicals present. The results revealed that Aspergillus austwickii contains phytoconstituents such as alkaloids, phenols, flavonoids, tannins, and carbohydrates. The total phenolic content and total flavonoid content of the fungus were found to be 22.048 µg GAE/g and 18.828 µg QE/g, respectively. The crude extract demonstrated 46.20±0.53% antioxidant activity, with an IC50 value of 128.69 µg/mL for radical scavenging as determined by the DPPH assay. It also exhibited 71.86±0.34% anti-inflammatory activity in the protein denaturation assay. Notably, it displayed antidiabetic activity against both α-amylase and α-glucosidase, with percentage inhibitions of 68.22±0.17% and 73.72±0.18%, and IC50 values of 178.10 µg/mL and 166.16 µg/mL, respectively. The current study indicates that the methanolic extract of Aspergillus austwickii possesses significant antioxidant, anti-inflammatory, and antidiabetic properties. The findings of this research can inform future studies aimed at discovering new natural drugs to combat various disorders.

荆芥叶内生曲霉的体外药理活性研究。
糖尿病是一种在世界范围内迅速蔓延的广泛发生的非传染性疾病。它是由血液中葡萄糖的失调或α-淀粉酶和α-葡萄糖苷酶的功能失调引起的。内生菌是寄生在植物活组织内的微生物。内生真菌具有合成多种重要生物活性代谢物的能力。本研究旨在探索从民族植物药用植物细叶芹中分离的较少报道的内生真菌曲霉(Aspergillus austwickii)。对该内生真菌的甲醇提取物进行体外抗氧化、抗炎和抗糖尿病试验,并分析其总酚和总黄酮含量以及植物化学物质的含量。结果表明,奥氏曲霉含有生物碱、酚类、黄酮类、单宁和碳水化合物等植物成分。总酚含量为22.048µg GAE/g,总黄酮含量为18.828µg QE/g。DPPH法测定其抗氧化活性为46.20±0.53%,IC50值为128.69µg/mL。蛋白变性实验显示抗炎活性为71.86±0.34%。对α-淀粉酶和α-葡萄糖苷酶的抑制率分别为68.22±0.17%和73.72±0.18%,IC50值分别为178.10µg/mL和166.16µg/mL。目前的研究表明,奥氏曲霉的甲醇提取物具有显著的抗氧化、抗炎和抗糖尿病的特性。这项研究的发现可以为未来的研究提供信息,旨在发现新的天然药物来对抗各种疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Archives of Razi Institute
Archives of Razi Institute Veterinary-Veterinary (all)
CiteScore
1.50
自引率
0.00%
发文量
108
审稿时长
12 weeks
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