Beneficial effects of doxycycline on atrial electrical remodelling in a rat model of atrial fibrillation.

IF 2.1 4区医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS

Acta cardiologica Pub Date : 2025-05-08 DOI:10.1080/00015385.2025.2500888

Kai Zhang, Zuowang Ma, Yu Yang, Guangping Li

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引用次数: 0

Abstract

Background: Previous studies showed that doxycycline (Dox) can attenuate chronic intermittent hypoxia (CIH)-induced atrial fibrosis in rats. On this basis, we further investigated the effects of Dox on CIH-induced atrial electrical remodelling.

Methods: Rats were randomised into 3 groups: Control group, CIH group, and CIH with Dox treatment (CIH-D) group (n = 30). CIH and CIH-D rats were subjected to CIH 8 h/d for 6 weeks. After collecting the basic parameters of the rats, atrial fibrillation (AF) inducibility, conduction inhomogeneity, and epicardial conduction velocity were examined by in vitro cardiac electrophysiology experiments. The expression levels of ion channel subunits in the atrium were detected by Western blotting. Whole-cell patch clamp experiments were used to recorded action potential (AP), I_Ca-L, I_to, and the kinetic parameters.

Results: Compared to the Control rats, CIH rats showed increased AF inducibility, conduction inhomogeneity, and expression levels of p-RyR2, p-CaMKII, K_v11.1, K_ir2.3, K_Ca3.1, whereas the epicardial conduction velocity, I_Ca-L, I_to, and expression levels of Ca_v1.2, K_v1.5, K_v4.3 were decreased. Dox-treatment significantly improved the expression levels of K_v1.5, K_v4.3 and K_ir2.3 in CIH-D rats.

Conclusion: CIH caused atrial electrical remodelling in our rats, which was improved by Dox treatment. These changes indicated the potential effects of Dox in AF.

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强力霉素对大鼠房颤模型心房电重构的有益作用。

背景：以往的研究表明，强力霉素（Dox）可以减轻慢性间歇性缺氧（CIH）诱导的大鼠心房纤维化。在此基础上，我们进一步研究了Dox对cih诱导的心房电重构的影响。方法：将大鼠随机分为3组：对照组、CIH组和CIH加Dox治疗（CIH- d）组（n = 30）。CIH和CIH- d大鼠给予CIH 8 h/d，连续6周。采集大鼠心房颤动基本参数后，采用体外心脏电生理实验检测心房颤动的诱导性、传导不均匀性和心外膜传导速度。Western blotting检测心房离子通道亚基的表达水平。采用全细胞膜片钳实验记录动作电位（AP）、ICa-L、Ito及动力学参数。结果：与对照组相比，CIH大鼠心房纤颤诱导性、传导不均匀性、p-RyR2、p-CaMKII、Kv11.1、Kir2.3、KCa3.1表达水平升高，心外膜传导速度、ICa-L、Ito及Cav1.2、Kv1.5、Kv4.3表达水平降低。dox处理显著提高了CIH-D大鼠Kv1.5、Kv4.3和Kir2.3的表达水平。结论：CIH引起大鼠心房电重构，阿霉素可改善心房电重构。这些变化表明阿霉素在房颤中的潜在作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta cardiologica 医学-心血管系统

CiteScore

2.50

自引率

12.50%

发文量

115

审稿时长

2 months

期刊介绍： Acta Cardiologica is an international journal. It publishes bi-monthly original, peer-reviewed articles on all aspects of cardiovascular disease including observational studies, clinical trials, experimental investigations with clear clinical relevance and tutorials.